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目的 :探讨蛋白激酶 C(PKC)在实验性蛛网膜下腔出血所致脑血管痉挛发生发展过程中的作用及意义。方法 :采用成年犬枕大池二次注血法建立脑血管痉挛动物模型 ,通过非放射性同位素标记蛋白激酶测定法动态测定犬脑基底动脉平滑肌细胞 PKC活性。结果 :动态脑血管造影显示 ,实验组 day4组和 day7组基底动脉口径分别为正常对照组基底动脉口径的 (73.8± 3.4) %和 (5 1.1± 2 .1) % ,与对照组比较差异显著 (P <0 .0 1) ;day4和day7组均有膜 PKC活性升高、胞浆 PKC活性相应降低 ,与对照组比较 day4组无显著差异 (P >0 .0 5 ) ,day7组差异显著 (P <0 .0 1) ,day 4和 day 7组之间差异显著 (P <0 .0 1)。结论 :脑血管痉挛过程中 PKC被持续激活 ,并在脑血管痉挛病理发展过程中起关键作用。
Objective: To investigate the role and significance of protein kinase C (PKC) in the development of cerebral vasospasm induced by experimental subarachnoid hemorrhage. Methods: The animal model of cerebral vasospasm was established by the secondary injection of adult canine pillow and the PKC activity of canine basilar artery smooth muscle cells was measured by non-radioactive isotope labeled protein kinase assay. Results: Dynamic cerebrovascular angiography showed that the caliber of basilar artery of day4 and day7 in experimental group were (73.8 ± 3.4)% and (51.1 ± 2.1)%, respectively, which were significantly different from those in control group (P <0.01). The activity of PKC in membrane was increased and the activity of PKC in cytoplasm decreased in day4 and day7 groups, but there was no significant difference between day4 and day7 (P> 0.05) (P <0.01). There was significant difference between day 4 and day 7 (P <0.01). CONCLUSIONS: PKC is activated continuously during cerebral vasospasm and plays a key role in the pathological development of cerebral vasospasm.