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目的了解慢性乙型肝炎时，Ｃ－反应蛋白（ＣＲＰ）在肝细胞中的表达。方法用本室建立的ＬＢＡ法检测４０例ＨＢｓＡｇ阳性慢性肝炎的肝组织和１５例ＨＢｓＡｇ阴性的正常肝组织的ＣＲＰ原位表达；用免疫双标记染色检测肝细胞中ＣＲＰ、ＨＢｓＡｇ的表达。结果４０例肝炎标本ＣＲＰ均呈阳性，表达位置和形态与ＨＢｓＡｇ相似；免疫双标记染色显示肝细胞中ＣＲＰ与ＨＢｓＡｇ有重叠。此外，Ｔ淋巴细胞也表达ＣＲＰ。结论ＣＲＰ可与ＨＢｓＡｇ形成复合物。ＣＲＰ可能与慢性乙型肝炎的发病机制有关
Objective To understand the expression of C-reactive protein (CRP) in hepatocytes in chronic hepatitis B patients. Methods The LPC method was used to detect the expression of CRP in 40 liver tissues of HBsAg positive chronic hepatitis and 15 normal HBsAg negative normal liver tissues. The expressions of CRP and HBsAg in hepatocytes were detected by double immunofluorescence staining. Results All 40 hepatitis C specimens were positive for CRP, and their location and morphology were similar to that of HBsAg. Double immunofluorescence staining showed that there was overlap of CRP and HBsAg in hepatocytes. In addition, T lymphocytes also express CRP. Conclusion CRP can form a complex with HBsAg. CRP may be related to the pathogenesis of chronic hepatitis B.