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目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因1 525位点G/A、1 595位点C/T单核苷酸多态性(SNP)与妊娠期糖尿病(GDM)的关系及TRAIL在GDM发生中的可能作用。方法:利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)的方法检测TRAIL基因1 525位点G/A、1 595位点C/T多态性,并测定空腹血糖(FPG)、空腹胰岛素(FINS)等生化指标。计算相应的基因型频率和等位基因频率,通过χ2检验和方差分析,分析TRAIL基因型和等位基因与GDM及各生化指标的相关性。结果:GDM组TRAIL基因型频率和等位基因频率高于正常妊娠组(分别为P<0.05和P<0.01);GDM组GA/CT、AA/TT基因型FPG、FINS均高于GG/CC基因型(P<0.05)。结论:TRAIL基因1 525位点G/A、1 595位点C/T单核苷酸多态性与妊娠期糖尿病密切相关,可能参与GDM的发病机制。
OBJECTIVE: To investigate the relationship between G / A and C / T SNPs at 1 525 and gestational diabetes mellitus (GDM) at tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) And the possible role of TRAIL in GDM. Methods: PCR-RFLP was used to detect G / A and C / T polymorphisms at 1 525 loci at 1 525 locus of TRAIL gene. The levels of fasting blood glucose (FBG) FPG), fasting insulin (FINS) and other biochemical indicators. The corresponding genotype frequency and allele frequency were calculated. The correlation between TRAIL genotypes and alleles and GDM and biochemical indexes was analyzed by Chi-square test and analysis of variance. Results: The frequencies of genotypes and alleles of TRAIL in GDM group were higher than those in normal pregnancy (P <0.05 and P <0.01, respectively). The levels of FPG and FINS in GA / CT and AA / TT genotypes in GDM group were higher than those in GG / CC Genotype (P <0.05). CONCLUSION: The SNPs of G / A at 1 525 and C / T SNPs at 1 595 of TRAIL gene are closely related to gestational diabetes mellitus, which may be involved in the pathogenesis of GDM.