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收集１９６０～１９９２年间不同地区，不同宿主及媒介分离的鼠疫菌４６２株进行营养需求及突变的研究，利用Ｌａｗｔｏｎ氏最小培养基进行初筛，所有菌株均能生长。在限定培养基上，应用影印与定量法同步在２８℃下进行培养，结果有９５％的菌株需要苯丙氨酸（Ｐｈｅ－）、甲硫氨酸（Ｍｅｔ－）和半胱氨酸（Ｃｙｓ－）。少数菌株出现了营养突变，形成四种营养依赖型：缬氨酸依赖（Ｖａｌ－）、异亮氨酸依赖（Ｉｌｅ－）、甘氨酸依赖（Ｇｌｙ－）和缬、甘氨酸联合依赖（Ｖａｌ－Ｇｌｙ－）；四种低营养型：苯丙氨酸不依赖（Ｐｈｅ＋）。甲硫氨酸不依赖（Ｍｅ＋）、半胱氨酸不依赖（Ｃｙｓ＋）和甲硫、半胱氨酸联合不依赖（Ｍｅｔ＋Ｃｙｓ＋）。同时发现，各种营养表型与菌株的宿主种类、生化特性、质粒的存在于否和毒力因子关系方面均无显著关系，只有Ｐｇｍ－菌株约５０％为营养依赖型。不同地区分离的菌株对氨基酸的需求不同，全省３０余个疫源具有１４种营养类型，因此鼠疫菌的营养需求在地理分布上存在差异。这些结果表明，鼠疫菌对氨基酸的营养需求是其长期存在于特定的生态环境所形成的个独立系统，是长期进化的结果，其营养表型可能与毒力的表达无直接的联系。
A total of 462 strains of Y. pestis isolated from different areas from 1960 to 1992 were studied for their nutritional requirements and mutations. All the strains were able to grow with the minimal medium of Lawton’s minimal culture. On limited medium, culturing was performed synchronously at 28 ° C by photolithography and quantification. As a result, 95% of the strains required phenylalanine (Phe-), methionine (Met-) and cysteine -). A few strains appeared nutritional mutation, forming four nutritionally dependent types: Val-, Ile-, Gly- and Val-Gly- ); Four low-nutrient: Phenylalanine is not dependent on (Phe +). Methionine is not dependent on (Me +), cysteine is not dependent on (Cys +) and methionine, and cysteine is not dependent on (Met + Cys +). At the same time, it was found that there was no significant relationship between the various nutritional phenotypes and host strains, biochemical characteristics, presence of plasmids and virulence factors. Only about 50% of Pgm-strains were nutritionally dependent. The strains isolated from different regions have different needs for amino acids. There are 14 types of nutrition in more than 30 epidemic areas in the province. Therefore, there are differences in the geographical distribution of Y. pestis. These results indicate that the nutritional requirement of Yersinia pestis is an independent system formed by its long-term existence in a specific ecological environment and is the result of long-term evolution. Its nutritional phenotype may not be directly related to the expression of virulence.