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通过登革病毒包膜蛋白基因序列测定与进化分析,确定2004 2010年间福建省登革病毒的主要来源地。方法提取登革病毒分离株的RNA,扩增病毒外膜蛋白基因,扩增产物经TA克隆后提取质粒并测序,应用相关专业软件做序列分析。结果在测定的12株登革病毒外膜蛋白基因核酸序列中,1、2型病毒的序列长度均为1 485bp,3型为1 479bp。BLAST比对与序列进化树均表明,2004-2010年期间,福建省所分离的登革病毒分离株与东南亚一带流行的毒株一致性最高,福建省与东南亚流行的毒株间具有高度的同源性。结论2004-2010年福建省流行的登革病毒应当是由东南亚一带输入,应加强对该地区入境人员的监测工作。
Through dengue virus envelope protein gene sequencing and evolutionary analysis to determine the main source of Dengue virus in Fujian Province in 2010 from 2004 to. Methods The RNA of dengue virus was extracted and the virus outer membrane protein was amplified. The amplified product was cloned and sequenced. The relevant software was used for sequence analysis. Results Among the 12 strains of dengue virus outer membrane proteins, the sequences of type 1 and 2 viruses were 1 485 bp in length and 1 479 bp in type 3, respectively. Both BLAST and sequence-based phylogenetic trees showed that the isolates of Dengue virus isolated in Fujian Province had the highest identity with the strains endemic to Southeast Asia from 2004 to 2010, and the strains with the highest prevalence among the strains endemic to Fujian and Southeast Asia Source. Conclusion The epidemic dengue virus in Fujian province should be imported from Southeast Asia in 2004-2010. Surveillance of immigrants in the area should be strengthened.