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利用DC Chol制备粒径为 5 0～ 30 0nm的正电荷脂质体 ,作为乙肝疫苗 (HBsAg)的佐剂 ,免疫BALB/c小鼠后进行血清中特异性抗体IgG1及IgG2a、脾细胞产生细胞因子的检测。结果该脂质体佐剂所诱导的抗体亚类以IgG2a为主 ,脾细胞产生的IL 2、IL 5、IFN γ分别比铝佐剂组高 16 5倍、 10倍、 2倍。表明该脂质体佐剂可以诱导很强的细胞免疫反应 ,是一种能促进Th1和Th2均衡应答的佐剂 ,值得作进一步的研究
DC Chol was used to prepare positive charge liposomes with the diameter of 50 ~ 300 nm as the adjuvant of hepatitis B vaccine (HBsAg), and the specific antibodies IgG1 and IgG2a in serum were obtained after immunization of BALB / c mice. The spleen cells Factor detection. Results The antibody subtypes induced by liposome adjuvant were mainly IgG2a. The spleen cells produced IL5, IL5 and IFNγ by 16 5 times, 10 times and 2 times higher than that of aluminum adjuvant respectively. This liposome adjuvant can induce a strong cellular immune response and is an adjuvant that can promote a balanced response between Th1 and Th2 and deserves further study