胆碱酯酶(ChE)活力测定是有机磷农药和神经性毒剂中毒的主要检验指标。它的测定方法很多,国內常用的是羟胺比色法和纸片法。前法所需时间较长,操作繁杂,后法太粗,结果难以判定。 Ellman等提出用乙酰硫代胆碱(AThch)作基质,二硫双硝基苯甲酸(DTNB)作显色剂的一种新方法称DTNB法,受到多方重视,国外日益广泛应用。但是,该法还沒有解决试剂溶液不稳定和血色素干扰等问题。为解决这些问题,我们参照有关资料对Ellman法作了一 Cholinesterase (ChE) activity determination of organophosphate pesticides and neurotoxic agents poisoning the main test indicators. Its determination of many ways, the domestic commonly used hydroxylamine colorimetry and paper method. The former method requires a long time, complicated operation, the method is too thick, the result is difficult to determine. Ellman et al. Proposed a new method of using DTh as a substrate with acetylthiocholine (AThch) as a substrate. The DTNB method has attracted much attention and has been widely applied abroad. However, this method has not solved the problems of unstable reagent solution and hemoglobin interference. To solve these problems, we made reference to the information on Ellman’s law