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目的设计准静态平面流场的灌注式新型生物反应器,以适合组织工程皮肤的培养要求。方法采用计算流体动力学(computational fluid dynamics,CFD)商业软件FLUENT模拟新型生物反应器中的流场及培养支架壁面上的切应力,设计出一种能够层叠、低剪切力、均匀平面流场的生物反应器。结果模拟结果表明筛网状支撑物及组织工程皮肤构建物表面处的最大切应力小于3×10-5N/cm2,且培养室中的流场是均匀的。结论新型生物反应器满足准静态平面流场的要求,有可能在未来的皮肤组织工程研究和生产中广泛使用。
Objective To design a new type of bioreactor for perfusion in a quasi-static planar flow field, which is suitable for the culturing of tissue engineering skin. Methods The computational fluid dynamics (CFD) commercial software FLUENT was used to simulate the flow field in a novel bioreactor and to cultivate the shear stress on the stent wall. A laminar flow, a low shear force, a uniform planar flow field Bioreactor. Results The simulation results showed that the maximum shear stress at the surface of the mesh support and the tissue engineered skin construct was less than 3 × 10 -5 N / cm 2, and the flow field in the culture chamber was uniform. Conclusion The new bioreactor meets the quasi-static planar flow field requirements and may be widely used in the future research and production of dermal tissue engineering.