论文部分内容阅读
选用栝楼的藤茎为原料,采用单因素试验和正交试验设计的方法,对栝楼藤茎多糖的微波辅助提取工艺进行优化;以DPPH自由基清除率、对羟自由基清除率和还原能力为指标,评价栝楼藤茎多糖的体外抗氧化活性。结果表明:微波辅助提取栝楼藤茎多糖的最佳工艺条件为:料液比为1:15(g:mL),微波处理时间为4 min,微波功率为700 W,此提取条件下栝楼藤茎多糖的平均得率为3.71%;抗氧化试验结果显示,清除DPPH自由基和羟自由基的半抑制浓度(IC_(50))分别为1.829 mg/mL和0.52 mg/mL;还原能力测定试验中,当栝楼藤茎多糖的质量浓度为2.5 mg/mL时,在700 nm下的吸光度值达到0.426。说明栝楼藤茎多糖具有抗氧化活性,具有进一步研究的价值。
In this paper, we selected the cane of Tuan Lou as the raw material, and optimized the microwave-assisted extraction process of cane polysaccharide from Tiandan Lou by single factor experiment and orthogonal experimental design. The radical scavenging rate and reduction of hydroxyl radical Ability as an index to evaluate the anti-oxidation activity of Cane sugar cane polysaccharides in vitro. The results showed that the optimum conditions of microwave-assisted extraction of cane polysaccharide were as follows: solid-liquid ratio 1:15 (g: mL), microwave treatment time 4 min and microwave power 700 W. Under this extraction condition, The average yield of cane polysaccharide was 3.71%. The results of antioxidant test showed that the half-inhibitory concentration (IC 50) of DPPH radical scavenging and hydroxyl radical scavenging were 1.829 mg / mL and 0.52 mg / mL, respectively. In the experiment, the absorbance value at 700 nm reached 0.426 when the mass concentration of cane polysaccharide in Tamarix was 2.5 mg / mL. That cinnabar cane polysaccharides with antioxidant activity, with further research value.