论文部分内容阅读
目的探讨FlaB-PCR检测方法对钩端螺旋体(钩体)检测的可行性。方法根据鞭毛蛋白B基因(FlaB)选择引物,与卫生行业标准推荐的引物G1/G2对比,进行特异性与灵敏度研究,并应用于龙游、常山两地的蛙、鼠标本钩体PCR检测。对浙江省2007年分离菌株进行钩体PCR鉴定。结果FlaB-PCR具有较高的灵敏度和特异性,该引物可特异性扩增致病性钩体DNA,对本实验所用的其他细菌不扩增。2007年分离的钩体菌株进行PCR鉴定结果与血清学鉴定结果相符。鼠和蛙肾标本进行FlaB-PCR检测,阳性率20.94%,与卫生行业标准推荐的引物G1/G2对比,两法符合率为90.54%。结论FlaB-PCR检测方法可灵敏、特异地检测致病性钩体,能正确有效地反映野生动物带病毒率,可为控制钩体病提供依据。
Objective To investigate the feasibility of FlaB-PCR for the detection of Leptospira (Leptospira). Methods The specificity and sensitivity of the primers were compared with those of the standard recommended by the health industry according to the FlaB flage primer selection. The PCR products were also used for PCR detection of the frogs and mice in Longyou and Changshan Mountains. Isolation of isolates from Zhejiang province in 2007 by PCR identification. Results FlaB-PCR has high sensitivity and specificity. This primer can specifically amplify the pathogenic leptospira DNA and not amplify the other bacteria used in this experiment. The isolates of Leptospira isolates from 2007 were identified by PCR and confirmed by serological identification. The positive rate of FlaB-PCR was 20.94%. Compared with the standard G1 / G2 recommended by the health industry, the coincidence rate of the two methods was 90.54%. Conclusion FlaB-PCR detection method can sensitively and specifically detect pathogenic leptospira, which can reflect the virus carrying rate of wild animals correctly and effectively, which can provide basis for controlling leptospirosis.