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目的:探讨不同剂量鼻用激素对变应性鼻炎(AR)小鼠鼻黏膜重塑及基质金属蛋白酶-9(MMP-9)表达的影响。方法:将30只雌性BALB/c小鼠随机分为5组,A组[生理盐水组(NS组)],B组[卵清蛋白组(OVA组)],D1组(低剂量布地奈德组),D2组(中剂量布地奈德组)及D3组(高剂量布地奈德组)。分别接受OVA或NS腹腔注射、鼻部激发。D1、D2及D3组小鼠使用不同剂量的布地奈德鼻喷激素(0.6μg/20g、3.0μg/20g、15.0μg/20g)喷鼻12周,1次/d。分别在4周末及16周末评估小鼠鼻部症状学的变化,并于16周末取小鼠鼻腔组织分别行苏木精-伊红染色、PAS及MT法染色评估其鼻腔黏膜重塑变化。使用ELISA测定小鼠鼻腔黏膜MMP-9的含量。结果:16周末B组小鼠的挠鼻、喷嚏次数及嗜酸粒细胞浸润个数均较A组增多,且出现杯状细胞增多,黏膜下腺体增生、肥大,上皮下纤维化及胶原沉积的变化。0.6μg/20g的布地奈德治疗可以抑制AR小鼠的鼻部症状学变化及嗜酸粒细胞浸润,而对于鼻腔黏膜重塑变化则需要至少3.0μg/20g的鼻喷激素。鼻喷激素可以抑制AR小鼠鼻腔中MMP-9的分泌。结论:高剂量鼻用激素可能通过抑制AR小鼠鼻腔MMP-9的分泌,抑制鼻腔黏膜重塑的发展。
Objective: To investigate the effects of different doses of nasal hormones on nasal mucosal remodeling and matrix metalloproteinase-9 (MMP-9) expression in allergic rhinitis (AR) mice. Methods: Thirty female BALB / c mice were randomly divided into five groups: group A (NS group), group B (OVA group), group D1 (low dose budesonide Group), group D2 (middle dose budesonide group) and group D3 (high dose budesonide group). OVA or NS were received intraperitoneal injection of NS, nasal excitation. D1, D2 and D3 mice were given nasal spray at different doses of budesonide (0.6μg / 20g, 3.0μg / 20g, 15.0μg / 20g) for 12 weeks once a day. Nasal symptomatology was assessed at the 4th and 16th week respectively. Nasal mucosa remodeling was assessed by hematoxylin-eosin staining and PAS staining at the end of 16th week. The content of MMP-9 in mouse nasal mucosa was measured by ELISA. Results: At the end of the 16th week, the numbers of flexing nose, sneezing and eosinophil infiltration in group B mice were more than those in group A, with the goblet cell proliferation, submucosal gland hyperplasia, hypertrophy, subcutaneous fibrosis and collagen deposition The change. Budesonide at 0.6 μg / 20 g inhibited nasal symptomatic changes and eosinophilic infiltration in AR mice, whereas nasal spray hormone required at least 3.0 μg / 20 g for nasal mucosal remodeling. Nasal spray hormones can inhibit the secretion of MMP-9 in the nasal cavity of AR mice. Conclusion: High-dose nasal hormones may inhibit the nasal mucosa remodeling by suppressing the secretion of MMP-9 in nasal cavity of AR mice.