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采用电化学法对自行合成的苯甲酸二聚铜配合物[Cu(R)2]与鲑鱼精DNA的相互作用进行了研究。配合物在0.197 V和0.162 V处有一对氧化还原峰。加入双链DNA(dsDNA)后,氧化还原峰电流明显降低且式量电位正移,说明苯甲酸二聚铜配合物与DNA以嵌入方式生成一种非电活性超分子复合物,导致苯甲酸二聚铜配合物的峰电流降低,峰电流在一定范围内与鲑鱼精dsDNA质量浓度呈线性关系,线性范围为3.0×10-2~4.5×10-3mg/L,检出限为3.5×10-4mg/L。同时,以[Cu(R)2]作杂交指示剂,把一种新型发夹结构锁核酸探针(LNA)固定在金电极表面制备了电化学生物传感器,将该修饰电极与人工合成的慢性粒细胞白血病BCR/ABL融合基因片段进行杂交,用差示伏安法进行检测,该铜配合物能够较好地区分探针序列、单碱基错配序列和互补链序列。
The interaction between self-synthesized dimeric copper benzoate [Cu (R) 2] and salmon sperm DNA was studied by electrochemical method. The complex has a pair of redox peaks at 0.197 V and 0.162 V. The addition of double-stranded DNA (dsDNA), the redox peak current decreased significantly and the potential volume-type shift, indicating that the benzoic acid dimeric copper complexes and DNA embedded to generate a non-electroactive supramolecular complexes, resulting in benzoic acid bis The peak current of poly (copper) complexes decreased and the peak current was linear with the concentration of dsDNA in salmon sperm. The linear range was 3.0 × 10-2 ~ 4.5 × 10-3mg / L with the detection limit of 3.5 × 10- 4mg / L. At the same time, an electrochemical biosensor was prepared by immobilizing a novel hairpin structure-locked nucleic acid probe (LNA) on the surface of gold electrode with [Cu (R) 2] as hybridization indicator. The myeloid leukemia BCR / ABL fusion gene fragment was hybridized and detected by differential voltage-voltammetry. The copper complex could distinguish the probe sequence, single-base mismatch sequence and complementary strand sequence well.