论文部分内容阅读
笔者在不同栖息场所采集Cella氏Ⅱ~Ⅲ期中华按蚊及嗜人按蚊共357只,同时用ELISA及沉淀试验鉴定蚊胃血中的人、牛血。结果357份标本,经沉淀试验人血阳性126份,占35.3%;牛血阳性59份,占16.5%。ELISA测定人血阳性135份,占37.8%;牛血阳性75份,占21.0%。后法较前法阳性率高,阳性反应强度较高。沉淀试验鉴定为人牛血的30份,ELISA测定人血5份,牛血8份,人牛血17份。沉淀试验鉴为猪血的130份,ELISA测定为阴性反应。沉淀试验鉴定人、牛血为阴性的12份标本,经ELISA法检测全为阳性反应,其中人4份,牛血8份,进一步证明后法较前法特异性强,更适合现场蚊胃血鉴定。
In this study, 357 Celosia anopheles and Anopheles anthropophagus were collected from different habitats, and the human and bovine blood in mosquito and stomach were identified by ELISA and sedimentation assay. Results 357 specimens, the precipitation test of human blood-126, accounting for 35.3%; bovine blood-positive 59, accounting for 16.5%. ELISA assay of human blood-135, accounting for 37.8%; bovine blood-positive 75, accounting for 21.0%. After the law than the positive rate of positive, high positive reaction intensity. Precipitation experiments identified as human bovine blood 30, 5 copies of human blood by ELISA, 8 parts of bovine blood, 17 parts of human bovine blood. Precipitation test for the pig blood of 130, ELISA assay was negative. Precipitation test identified people, bovine blood was negative in 12 specimens, all detected by ELISA positive reaction, of which 4 were in cattle, 8 copies of bovine blood, to further prove that the latter method is more specific than the former method, more suitable for on-site mosquito blood identification .