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目的：研究尿激酶型纤溶酶原（ｕ Ｐ Ａ） 激活系统中４ 种主要成分ｕ Ｐ Ａ、 Ｐ Ａ Ｉ１ 、 Ｐ Ａ Ｉ２ 、ｕ Ｐ Ａ Ｒ 在非小细胞性肺癌细胞株中的表达及各因子之间的作用方式。方法：应用免疫组化、蛋白免疫印迹技术对培养细胞中４ 种成分进行定位、定性及半定量分析；应用斑点杂交技术检测４ 种细胞中ｕ Ｐ Ａ ｍ Ｒ Ｎ Ａ 和ｕ Ｐ Ａ Ｒｍ Ｒ Ｎ Ａ 的表达。结果：４ 种成分在４ 株细胞的胞浆及胞膜均有表达。蛋白免疫印迹法检测ｕ Ｐ Ａ、 Ｐ Ａ Ｉ１ 、 Ｐ Ａ Ｉ２ 、ｕ Ｐ Ａ Ｒ，４ 株细胞均在约１４０ ｋｕ 和约８０ ｋｕ 处出现特异性条带，且两种腺癌细胞的表达水平显著高于其他两株细胞。斑点杂交结果示４ 株细胞中均有ｕ Ｐ Ａ ｍ Ｒ Ｎ Ａ 和ｕ Ｐ Ａ Ｒ ｍ Ｒ Ｎ Ａ 的表达。结论：此４ 株细胞中均能合成与分泌ｕ Ｐ Ａ 系统的４ 种主要成分，各因子以二联及三联复合物（ｕ Ｐ Ａ／ Ｐ Ａ Ｉ１ 、ｕ Ｐ Ａ／ Ｐ Ａ Ｉ２ 、ｕ Ｐ Ａ／ｕ Ｐ Ａ Ｒ、ｕ Ｐ Ａ／ Ｐ Ａ Ｉ１／ｕ Ｐ Ａ Ｒ、ｕ Ｐ Ａ／ Ｐ Ａ Ｉ２／ｕ Ｐ Ａ Ｒ） 的形式存在；ｕ Ｐ Ａ 系统的表达与这４ 种细胞的恶性程度并不一致。
OBJECTIVE: To study the four main components of uPA, PAI-1, PAI2, and uPAR in the non-small cell lung cancer cell line in the urokinase-type plasminogen (u PA) activation system. The expression and the mode of action between the various factors. METHODS: Immunohistochemistry and Western blotting techniques were used to locate, qualitatively and semi-quantitatively analyze the 4 components in the cultured cells; Dot-blot hybridization was used to detect u P A m R N A and u P A Rm R N in 4 cells. A’s expression. RESULTS: Four components were expressed in the cytoplasm and membrane of four cells. Western blotting showed that u PA, PA IL-1, PA IL-2, u P A R, and 4 cells all showed specific bands at about 140 ku and about 80 ku, and both adenocarcinoma cells The expression level was significantly higher than the other two cells. Dot blot results showed that the expression of u p A m R N A and u P A R m R N A in all 4 cells. Conclusion: The four major components of the u PA system can be synthesized and secreted in these 4 cells. The factors are bi- and triple-complexes (u P A / P A I -1, u P A / P A I - 2, u P A/u P A R, u P A/ P A I 1/ U P A R, u P A/ P A I 2/ u P A R) Form exists; u P A system The expression was not consistent with the degree of malignancy of the 4 cells.