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[目的]探讨雌激素对高氧所诱发新生大鼠肺损伤的影响.[方法]选用生后2d、体重为5～10g的新生Wistar大鼠,雌雄不拘,随机分为空气对照组、高氧模型组及雌激素治疗组.将空气对照组新生大鼠暴露在室内空气中,其他各组新生大鼠暴露在90%(体积分数)氧中共14d,雌激素治疗组新生大鼠每日分别腹腔内注射给予雌激素2mg/kg,空气对照组和高氧模型组腹腔内注射给予生理盐水.实验开始第3,7,14天时间点记录各组新生大鼠自然死亡数目,计算死亡率;每组处死10只,取肺组织,在光学显微镜下观察肺组织的病理变化,采用免疫组织化学染色方法观察肺组织中γ干扰素(IFN-γ)的表达.[结果]随着高氧暴露时间的延长,高氧模型组及雌激素治疗组死亡率均呈增高趋势,高氧模型组暴露7 d后开始死亡率迅速升高,而雌激素治疗组死亡率明显降低,相比较差异具有统计学意义(P<0.05).空气对照组IFN-γ无阳性表达,而高氧模型组中的阳性表达在第7天时达到高峰,第14天时明显下降,但仍高于空气对照组;雌激素治疗组中的IFN-γ表达强度在各时间段均明显低于高氧模型组(P<0.01).[结论]雌激素对新生大鼠高氧肺损伤有保护作用.
[Objective] To explore the effect of estrogen on lung injury induced by hyperoxia in neonatal rats. [Methods] Newborn Wistar rats weighing 5 ~ 10 g 2 days after birth were randomly divided into three groups: air control group, Model group and estrogen treatment group.The newborn rats in the air control group were exposed to the indoor air, the other groups were exposed to 90% (volume fraction) of oxygen for 14 days in total, and the estrogen-treated groups were given intraperitoneal The rats in the air control group and the hyperoxia group were given intraperitoneal injection of normal saline, and the number of natural deaths in each group was recorded on the 3rd, 7th and 14th days after the start of the experiment to calculate the death rate. Each time The rats in each group were sacrificed and the lungs were removed. The pathological changes of lung tissues were observed under light microscope. The expression of IFN-γ in lung tissues was observed by immunohistochemical staining. [Results] With the duration of hyperoxia exposure , The mortality rate in hyperoxia model group and estrogen treatment group showed an increasing trend. The mortality rate in hyperoxia model group increased rapidly at 7 days after exposure, while the estrogen treatment group mortality rate was significantly lower than that in the control group Significance (P <0.05). Air control The positive expression of IFN-γ in hyperoxia model group peaked on the 7th day and decreased significantly on the 14th day, but still higher than that in the air control group. The expression of IFN-γ in the estrogen treatment group was Each time period was significantly lower than the hyperoxia model group (P <0.01). [Conclusion] Estrogen has a protective effect on hyperoxia - induced lung injury in neonatal rats.