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国内文献未见对大鼠睾丸生精细胞凋亡检测和流式细胞术（FCM）研究的报道。本实验选用正常雄性SD大鼠10只，体重250－300克，剖杀后取睾丸组织进行FCM检测、荧光显微镜检测等研究。FCM=维细胞图谱显示：生精细胞前向散射值较高，侧向散射值较低；另还显示出三群生精细胞群，其中以低值散射的小细胞群密度最大。FCM倍体分析及周期分析图证实，高密度的小细胞群为单倍体生精细胞．另两群体积较大的细胞分别为双倍体及四倍体生精细胞。本实验较成功地建立了正常大鼠睾丸生精细胞FCM倍体分析及增殖周期图。与knudson等（Science，1995；270：96）报道的正常小鼠FCM分析图相比较，大、小鼠都以单倍体生精细胞的FCM峰值最高。但我们的研究发现：大鼠双倍体生精细胞FCM峰值较小鼠为低，而四倍体生精细胞峰值较小鼠稍高。大鼠生精细胞经PI染色后荧光显微镜观察，检见少数强激发荧光的调亡生精细胞。睾丸切片HE染色后光镜观察，曲细精管中可见少数散在的凋亡生精细胞。实验通过对大鼠生精细胞FCM分析和对生精细胞凋亡检测的研究，为生殖毒理学研究和抗生育药物诱导生精细胞凋亡建立了较好的大鼠模型。我们近期进行的吗啡成德大鼠实验模型的研究中，用FCM检测到吗啡诱导生精细胞凋亡的低值单倍体凋亡峰，用PI荧光?
There is no report in domestic literatures on the testicular spermatogenic cell apoptosis test and flow cytometry (FCM) in rats. In this experiment, 10 normal male SD rats weighing 250-300 g were used. FCM test and fluorescence microscope test were used to test the testis tissue. FCM = dimensional cytometry showed that the spermatogenic cells had higher forward scattering value and lower lateral scattering value, and also showed three groups of spermatocyte population, of which the density of small cell population scattering at low value was the highest. FCM analysis of ploidy and cycle analysis confirmed that high density of small cell populations for the haploid spermatogenic cells. The other two groups of larger cells were diploid and tetraploid spermatogenic cells. In this experiment, we successfully established FCM ploidy analysis and proliferation cycle diagram of spermatogenic cells of normal rats. In comparison with the normal mouse FCM profile reported by knudson et al. (Science, 1995; 270: 96), the peak of FCM of haploid spermatogenic cells was the highest in both large and mouse. However, our study found that the peak of tetraploid spermatogenic cell FCM in rats was lower than that in mice and the peak of tetraploid germ cells was slightly higher than that in mice. The spermatogenic cells of rats were stained with PI and observed under a fluorescence microscope. A few of the apoptotic spermatogenic cells with strong excitation fluorescence were detected. After HE staining, the testicular sections were observed under light microscope. A few scattered apoptotic spermatogenic cells were observed in seminiferous tubules. In this study, FCM analysis of spermatogenic cells in rats and the detection of apoptosis of spermatogenic cells were used to establish a better rat model for reproductive toxicology research and anti-fertility drug-induced apoptosis of spermatogenic cells. In our recent study on morphine-induced rat model, morphine-induced apoptotic apoptotic haploidentical apoptotic peak was detected by FCM.