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随着电子显微镜在生物医学研究中的广泛应用,对所观察样品的制备条件的要求也越来越严格。自从Watson(1958)首先提出铅化合物可以增加超薄切片中细胞超微结构的反差以来,目前国内外多采用Reynolds(1963)介绍的柠檬酸铅作为常规铅染液。但多年来,超薄切片的铅污染却是许多实验室普遍存在的问题,它直接影响着切片的质量和电镜观察效果,原因在于过去所用的铅染液在接触空气中的二氧化碳以后常产生碳酸铅沉淀,污染切片。而且铅染液不能长期贮存,否则污染会更加严重。为了解决这个问题,我们参照Hanaichi等(1986)改进的铅数染液配方和染色方法进行染色。获得了满意的结果,特报告如下。
With the widespread use of electron microscopy in biomedical research, the requirements for the conditions under which the samples are observed are becoming more stringent. Since Watson (1958) firstly proposed that lead compounds can increase the ultrastructure of cells in ultrathin sections, the lead citrate solution introduced by Reynolds (1963) is widely used at home and abroad. However, lead contamination of ultrathin sections has been a common problem in many laboratories for many years. It directly affects the quality of sliced sections and the effect of electron microscopy. The reason for this is that lead used in the past often produces carbonic acid after exposure to carbon dioxide in the air Lead precipitation, pollution slice. And lead-free liquid can not be long-term storage, otherwise the pollution will be more serious. In order to solve this problem, we refer to Hanaichi et al (1986) improved lead number dye solution and staining method for staining. Satisfied with the results, special report is as follows.