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目的研究新近报道与丙氨酸转氨酶异常相关的ＴＴＶ在已知和未知病毒性肝炎中的临床意义。方法设计ＴＴＶ部分基因的特异性引物，用聚合酶链反应（ＰＣＲ）方法检测了１０４例病毒性肝炎的ＴＴＶＤＮＡ，并对１例ＴＴＶ阳性标本克隆测序。结果ＴＴＶＤＮＡ序列与日本ＴＴＶ部分基因序列相对应位置的核苷酸同源性为９８．４％。在１０４例肝炎患者中ＴＴＶＤＮＡ阳性检出率为２４．０％（２５／１０４），其中在非甲～戊和庚型肝炎患者中为４８．０％（１２／４２），在甲型肝炎中为１９．０％（４／２１），乙型肝炎为２５．０％（８／３２），丙型肝炎为１１．１％（１／９），９例ＨＧＶＲＮＡ阳性者中未检出ＴＴＶＤＮＡ。值得注意的是重型肝炎ＴＴＶ检出率极高，其中急性重型肝炎６例中阳性为４例（６６．７％），慢性重型肝炎６例中阳性为３例（５０．０％）。结论ＴＴＶ在我国肝炎病人中存在。在重型肝炎中有较高的发生率，可能是未知病毒致急性和慢性及重型肝炎的病因之一。
Objective To study the clinical significance of newly reported TTV associated with alanine aminotransferase abnormalities in known and unknown viral hepatitis. Methods The specific primers of some TTV genes were designed and the TTV DNA of 104 cases of viral hepatitis were detected by polymerase chain reaction (PCR). One case of TTV positive clone was sequenced. Results The nucleotide homology of TTTVNA sequence to the corresponding part of Japanese TTV gene sequence was 98.4%. The positive rate of TTVDNA in 104 hepatitis patients was 24.0% (25/104), of which 48.0% (12/42) in non-hepatitis E and G hepatitis patients, Were 19.0% (4/21), 25.0% (8/32) in hepatitis B and 11.1% (1/9) in hepatitis C. No TTVDNA was detected in 9 cases of HGV RNA positive. It is worth noting that the detection rate of TTV in patients with severe hepatitis is extremely high, among which, 6 cases of acute severe hepatitis were positive in 4 cases (66.7%) and 6 cases of chronic severe hepatitis were positive in 3 cases (50.0%). Conclusions TTV is present in hepatitis patients in China. A high incidence in severe hepatitis may be one of the causes of acute and chronic and severe hepatitis in unknown viruses.