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在酶联免疫吸附试验(ELISA)中,聚苯乙烯反应板的有效吸附率仅10％,而且这种反应板常因生产批号及质量不同,试验中易出现本底高或边缘效应。为此,1979年Towbin等人用硝酸纤维素腆取代聚苯乙烯反应板,改进了酶联免疫吸附试验,建立了斑点酶联免疫吸附试验(Dot-ELISA)。目前国外已将其应用于烟草花叶病毒、腺病毒、绵羊肝片吸虫等各种疾病的诊断和流行病调查。 1986年,我们应用斑点酶联免疫吸附试验,将自己获得的抗黄瓜花叶病毒单克隆抗
In enzyme-linked immunosorbent assay (ELISA), the effective adsorption rate of polystyrene reaction plate is only 10%, and the reaction plate is often due to production lot number and quality, the test is prone to high background or edge effect. Toward this end, Towbin et al. Replaced the polystyrene plate with nitrocellulose 1979 in 1979 to improve the enzyme-linked immunosorbent assay (ELISA) and establish Dot-ELISA. At present, it has been applied to the diagnosis and epidemic investigation of various diseases such as tobacco mosaic virus, adenovirus and fistula in sheep. In 1986, we applied the dot blot enzyme-linked immunosorbent assay to clone the monoclonal anti-cucumber mosaic virus