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雷蒙德氏棉基因组草图的完成为棉花的基础研究奠定基础,然而该基因组草图仍需要后续的补充和完善。利用雷蒙德氏棉基因组草图信息,分别在6条较长染色体的端部选取了4 kb的序列。通过生物信息学分析这些序列在基因组中的拷贝情况,发现2号染色体一端的序列Chr2D属于单拷贝序列,且其内部没有重复序列。随后以该段序列为探针,对雷蒙德氏棉有丝分裂中期染色体进行荧光原位杂交,结果显示在1号染色体和4号染色体的一端有明显的杂交信号,信号大小远远大于4 kb序列所能产生信号的大小,而信号的强度则比正常的杂交信号暗弱。试验结果说明该4 kb序列Chr2D可能在1号染色体和4号染色体的1个端部有较高的拷贝数,而信号强度的暗弱则说明该序列在染色体上的分布方式可能为散漫分布。杂交信号在1号染色体和4号染色体上的相似性说明,这2对染色体可能有一定的亲缘关系。该结果将对雷蒙德氏棉基因组草图的补充完善有帮助。
Completion of the Raymond cotton genome mapping has laid the foundation for basic cotton research, yet the draft genome still needs to be supplemented and refined. Using the DNA of the genome of Raymond Cotton, we selected a 4 kb sequence at the end of 6 longer chromosomes. The bioinformatics analysis of the copy number of these sequences in the genome revealed that Chr2D, a sequence on chromosome 2, belongs to a single-copy sequence and has no internal repeats. Then, using this sequence as a probe, fluorescence in situ hybridization of mitotic metaphase chromosomes of Raymond cotton showed that there was a significant hybridization signal at the end of chromosome 1 and chromosome 4, and the signal size was much larger than the 4 kb sequence The size of the signal that can be produced, and the intensity of the signal is weaker than the normal hybridization signal. The results showed that the 4 kb Chr2D gene may have a higher copy number on one end of chromosome 1 and 4, while the weak signal intensity indicates that the distribution of this sequence on chromosome may be scattered. The similarity of the hybridization signals on chromosome 1 and chromosome 4 indicates that the two pairs of chromosomes may have a certain genetic relationship. This result will be helpful for complementing the Raymond cotton genome sketch.