OBJECTIVE:To investigate the underlying mecha-nism of the effect of Fengreqing oral liquid(风热清口服液,FOL) on wind-heat pattern (WHP).METHODS:In this study,we predicted the poten-tial targets of FOL via the approach of network pharmacology and verified it by in vitro inflamma-tion model.In the network pharmacology part,two strategies,namely the direct target search and the indirect one,were used to collect the target sets of FOL in WHP treatment.The enrichment analysis was carried out by David database and ClueGo plug-in in Cytoscape.Furthermore,the potential targets were mapped in the candidate pathways.In the verification experiment section,in vitro model of lipopolysaccharide (LPS) induced RAW 264.7 was used to confirm the predictive results in the net-work pharmacology part.RESULTS:Through the two screening strategies,a total of 141 non-repetitive intervention targets of FOL on WHP were obtained.Gene ontology (GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis showed that the inter-vention effect was mainly focused on the anti-in-flammatory effect,and the Toll-like receptor signal-ing pathway was one of the most critical regulatory pathways.Further mapping analysis showed that phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT) signaling transfer might be the key part of regulating the concentration of inflammation medi-ators of FOL in the Toll-like receptor signaling path-way.In vitro experiment showed that FOL signifi-cantly reduced the levels of NO,IL-1,IL-6,and TNF-α produced by RAW264.7 induced by LPS.Fur-ther immunofluorescence found that this effect is related to the regulation of PI3K-AKT pathway activ-ity by FOL.CONCLUSION:FOL can intervene in WHP by regu-lating the content of inflammatory mediators via the PI3K-AKT pathway.