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[目的]探讨厄多司坦对脂多糖(LPS)诱导的小鼠急性肺损伤(ALI)小鼠肺内抗氧化转录因子Nrf2表达的影响.[方法]健康雄性C57bl/6小鼠24只随机分为对照组、ALI组和厄多司坦组,每组8只.采用气管注射LPS(5mg/kg)复制小鼠ALI动物模型.厄多司坦组通过提前30min灌胃(150mg/kg)给予预处理,造模12h后处理小鼠.HE染色观察小鼠肺组织形态学改变;检测丙二醛(MDA)含量和超氧歧化物(SOD)活性反映肺组织氧化应激;Western blot法检测肺组织核内Nrf2的表达;Real-time PCR检测肺组织hO-1和SOD mRNA含量.[结果]厄多司坦可改善LPS诱导的ALI小鼠肺组织病理形态学改变,降低肺组织内MDA含量,而增加SOD活性.厄多司坦亦可显著性增加ALI小鼠肺组织细胞核内Nrf2的表达,并显著增加Nrf2下游靶基因HO-1和SOD mRNA的表达.[结论]厄多司坦能减轻LPS诱导的小鼠ALI,通过增强ALI小鼠肺内抗氧化转录因子Nrf2的核转位及转录活性可能是其机制所在.“,”[Objective]To investigate the effect of erdosteine on the expression of antioxidant transcription fac-tor Nrf2 in lung of acute lung injury(ALI)mice induced by lipopolysaccharide(LPS).[Methods] The healthy male C57bl/6 mice were randomly divided into control group,ALI group and erdosteine group(n=8).The mouse model of ALI was reproduced by intratracheal injection of LPS(5 mg/kg).Erdosteine(150 mg/kg)was given 30 min before delivery and the mice were sacrificed 12 h later.HE staining was used to observe the morphological changes of lung tissue in mice.The content of malondialdehyde(MDA)and superoxide dismutase(SOD)were measured to reflect the oxidative stress in lung tissue.The expression of Nrf2 in lung tissue was detected by West-ern blot.Real-time PCR was used to detect the contents of HO-1 and SOD mRNA in lung tissue.[Results]Er-dosteine could improve the histopathological changes of lung tissue induced by LPS in ALI mice,decrease the con-tent of MDA in lung tissue and increase the activity of SOD.Erdosteine could also significantly increase the expres-sion of Nrf2 in the nucleus of ALI mice and significantly increase the expression of HO-1 and SOD mRNA.[Con-clusion]Erdosteine can alleviate LPS-induced ALI in mice.The mechanism may be due to the enhancement of the nuclear translocation and transcription activity of the antioxidant transcription factor Nrf 2 in the lung of ALI mice.