AIM:To investigate the effect of two-channel gastric electrical stimulation(GES) with trains of pulses on gastric emptying and slow waves.METHODS:Seven dogs implanted with four pairs of electrodes and equipped with a duodenal cannula were involved in this study.Two experiments were performed.The f irst experiment included a series of sessions in the fasting state with trains of short or long pulses,each lasted 10 min.A 5-min recording without pacing was made between two sessions.The second experiment was performed in three sessions(control,single-channel GES,and two-channel GES) .The stimulus was applied via the 1st pair of electrodes for single-channel GES(GES via one pair of electrodes located at 14 cm above the pylorus) ,and simultaneously via the 1st and 3rd channels for two-channel GES(GES via two pairs of electrodes located at 6 and 14 cm above the pylorus) .Gastric liquid emptying was collected every 15 min via the cannula for 90 min.RESULTS:GES with trains of pulses at a pulse width of 4 ms or higher was able to entrain gastric slow waves.Two-channel GES was about 50% more efficient than single-channel GES in entraining gastric slow waves.Two-channel but not single-channel GES with trains of pulses was capable of accelerating gastric emptying in healthy dogs.Compared with the control session,two-channel GES signif icantly increased gastric emptying of liquids at 15 min(79.0% ± 6.4% vs 61.3% ± 6.1%,P < 0.01) ,30 min(83.2% ± 6.3 % vs 68.2% ± 6.9%,P < 0.01) ,60 min(86.9% ± 5.5 % vs 74.1% ± 5.9%,P < 0.01) ,and 90 min(91.0% ± 3.4% vs 76.5% ± 5.9%,P < 0.01) .CONCLUSION:Two-channel GES with trains of pulses accelerates gastric emptying in healthy dogs and may have a therapeutic potential for the treatment of gastric motility disorders. AIM: To investigate the effect of two-channel gastric electrical stimulation (GES) with trains of pulses on gastric emptying and slow waves.METHODS: Seven dogs implanted with four pairs of electrodes and equipped with a duodenal cannula were involved in this study.Two experiments were performed. f irst experiment included a series of sessions in the fasting state with trains of short or long pulses, each lasted 10 min. A 5-min recording without pacing was made between two sessions. second time was performed in three sessions (control, single-channel GES, and two-channel GES). The stimulus was applied via the 1st pair of electrodes for single-channel GES (GES via one pair of electrodes located at 14 cm above the pylorus), and simultaneously via the 1st and 3rd channels for two-channel GES (GES via two pairs of electrodes located at 6 and 14 cm above the pylorus). Gastric liquid emptying was collected every 15 min via cannula for 90 min .RESULTS: GES with trains of pulses at a pulse width of 4 ms or higher was able to entrain gastric slow waves.Two-channel GES was about 50% more efficient than single-channel GES in entraining gastric slow waves.Two-channel but not single-channel GES with trains of pulses was capable of accelerating gastric emptying in healthy dogs. Compared with the control session, two-channel GES signif icantly increased gastric emptying of liquids at 15 min (79.0% ± 6.4% vs 61.3% ± 6.1%, P <0.01) (91.9% ± 5.5% vs 74.1% ± 5.9%, P <0.01), and 90 min (91.0% ± 3.4% vs 76.5% ± 5.9%, P <0.01) %, P <0.01). CONCLUSION: Two-channel GES with trains of pulses accelerates gastric emptying in healthy dogs and may have a therapeutic potential for the treatment of gastric motility disorders.