论文部分内容阅读
用人神经母细胞瘤SH-SY5Y细胞系作为研究对象,通过测定细胞存活率(MTT法)和脂质过氧化代谢产物丙二醛(MDA),探讨了氯化钾(KCI)或谷氨酸分别对人神经母细胞瘤SH-SY5Y细胞的损伤作用。结果发现在含Ca~(-2)的温育液中,SH-SY5Y细胞与KCl(40-80mmol/L)或谷氨酸(50-100mmol/L)一起温育4h后,细胞存活率显著降低,脂质过氧化程度明显增高。当温育液中无Ca~(2+)时,KCl或谷氨酸均未引起细胞存活率和脂质过氧化程度的显著变化。相差显微镜观察细胞形态的变化与上述结果吻合,提示氯化钾或谷氨酸分别对SH-SY5Y细胞均有损伤作用,并且这种作用与细胞外Ca~(2+)内流和脂质过氧化有关。该传代细胞模型为筛选神经保护药提供了一种快速而简便的手段。
Using human neuroblastoma SH-SY5Y cell line as a research object, potassium chloride (KCI) or glutamic acid was investigated by measuring cell viability (MTT method) and lipid peroxidation product malondialdehyde (MDA). Injury of human neuroblastoma SH-SY5Y cells. The results showed that in the incubation medium containing Ca~(-2), the survival rate of SH-SY5Y cells was significantly improved after incubation with KCl (40-80 mmol/L) or glutamic acid (50-100 mmol/L) for 4 h. Lowered, the degree of lipid peroxidation increased significantly. When Ca~(2+) was absent in the incubation solution, neither KCl nor glutamic acid caused a significant change in cell viability and lipid peroxidation. Contrast microscope observation of cell morphology changes coincides with the above results, suggesting that KCl or glutamic acid respectively have a damaging effect on SH-SY5Y cells, and this effect is associated with extracellular Ca~(2+) influx and lipids. Oxidation related. This continuous cell model provides a quick and easy method for screening neuroprotective drugs.