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对正红菇纯培养RL1菌株做了多种营养生理试验研究，结果表明：适宜的碳源为蔗糖、甘露糖、葡萄糖，不能利用羧甲基纤维素钠；适宜的氮源为酵母膏、牛肉膏和蛋白陈，硫酸铵也能被较好地利用；适宜的C／N为20／1；矿质元素P，K，Mg对菌丝生长的影响很大，其中P的影响最显著，Ca和Na的影响很小；维生素肌醇对菌丝生长有明显的促进作用。
The results showed that suitable carbon sources were sucrose, mannose and glucose, and sodium carboxymethylcellulose could not be used. Suitable nitrogen sources were yeast extract, beef Paste and protein Chen, ammonium sulfate can also be better use; suitable C / N is 20/1; mineral elements P, K, Mg on mycelial growth is very large, of which the most significant impact of P, Ca and The effect of Na is very small; vitamin myo-inositol has a significant role in promoting mycelial growth.