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目的:探讨系统性红斑狼疮(systemlupus erythematosus,SLE)患者皮损血管内皮生长因子受体FLT-1和KDR水平的测定对疾病活动性及治疗转归的预测价值。方法:采用免疫组化(SP免疫组织化学法)法检测皮损FLT-1及KDR蛋白的表达水平,分析FLT-1和KDR的含量与SLE患者的疾病活动度指数SLEDAI和免疫指标(ANA、ds DNA等)的相关性,研究用FLT-1和KDR指标判断疾病的活动性,并进行SLE病例的前瞻性和治疗前后对照研究。结果:SLE患者皮损血管内皮生长因子受体FLT-1和KDR水平显著高于正常对照组。SLE患者皮损血管内皮生长因子受体FLT-1和KDR水平活动期均高于非活动期(P<0.01),抗核抗体(ANA)高滴度组高于ANA低滴度组,dsDNA抗体阳性组高于dsDNA抗体阴性组,肾损害组高于非肾损害组;且SLE患者皮损血管内皮生长因子受体FLT-1和KDR水平与SLE患者SLEDAI呈正相关。结论:FLT-1和KDR与SLE发病有关,可反映SLE患者的疾病活动性,同时定量检测可有助于预测患者的病情活动和转归。
Objective: To investigate the predictive value of the determination of FLT-1 and KDR in the lesions of patients with systemic lupus erythematosus (SLE) on the disease activity and the outcome of treatment. Methods: The expressions of FLT-1 and KDR protein in skin lesions were detected by immunohistochemistry (SP immunohistochemical method). The levels of FLT-1 and KDR were compared with those of SLE patients with disease activity index (SLEDAI) and immune indexes (ANA, ds DNA, etc.), the study used FLT-1 and KDR indicators to determine the activity of the disease, and SLE cases were prospective and controlled study before and after treatment. Results: The levels of FLT-1 and KDR in skin lesions of SLE patients were significantly higher than those in normal controls. The activities of FLT-1 and KDR in the skin lesions of SLE patients were significantly higher than those of the inactive ones (P <0.01). The high-titer anti-nuclear antibody (ANA) Positive group was higher than that of negative group of dsDNA antibody, and renal damage group was higher than non-renal damage group. The levels of FLT-1 and KDR in skin lesions of SLE patients were positively correlated with SLEDAI in SLE patients. Conclusion: FLT-1 and KDR are related to the onset of SLE and reflect the disease activity of patients with SLE. Simultaneously, quantitative detection of FLT-1 and KDR may be helpful to predict the patient’s condition and prognosis.