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盆栽香蕉及大蕉在人工降温至1℃时大蕉生长正常,经多效唑处理的香蕉呈轻度冷害而不处理的香蕉产生重度冷害。降温过程大蕉的过氧化物酶活性高于香蕉并保持稳定状态。香蕉的过氧化物酶活性有较大的波动,冷害出现时活性较低。经多效唑处理的香蕉在降温过程中过氧化物酶活性明显强于不处理的香蕉并保持稳定。香蕉与大蕉叶片中的过氧化物酶同工酶的酶谱类型不同。香蕉经降温处理后有若干酶带消失或活性减弱,大蕉在降温过程中既有酶带消失或活性减弱,亦有新酶带的增加或原酶带的活性增强。经多效唑处理的香蕉在降温过程中比不处理香蕉少消失一条酶带。本文对香蕉、大蕉耐冷力的差异与过氧化物酶的活性及同工酶的关系进行了讨论。
Plantain banana and plantain in the artificial cooling to 1 ℃ normal plantain growth, the paclobutrazol treatment banana mild cold damage without treatment of bananas have severe chilling injury. During the cooling process, plantain peroxidase activity was higher than that of banana and remained steady. Banana peroxidase activity has greater volatility, chilling injury appears to be less active. After the paclobutrazol treatment, the peroxidase activity of bananas was significantly stronger than that of non-treated bananas and remained stable. Enzymatic profiles of peroxidase isoenzymes in banana and plantain leaves are different. After banana was cooled down, some of the enzyme bands disappeared or the activity was weakened. During the cooling process, both the banana plantase disappeared or the activity weakened, and the increase of the new enzyme band or the activity of the original band was also observed. The paclobutrazol-treated bananas lost less of an enzyme band during the cooling process than non-treated bananas. In this paper, the differences between cold tolerance of banana and plantain, the activity of peroxidase and the isozyme were discussed.