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目的探讨不同糖耐量人群血浆25-羟维生素D[25(OH)D]与葡萄糖转运子4(GluT-4)及胆固醇调节元件结合蛋白1C(SREBP-1C)表达的关系。方法随机分为T2DM组、IGR组及正常对照(NGT)组。检测血糖、血脂、HbA1 c等,电化学发光法测定25(OH)D和FIns。Western blot检测GluT-4及SREBP-1C蛋白的表达,RT-PCR检测GluT-4和SREBP-1C mRNA的表达。结果T2DM组、IGR组25(OH)D低于NGT组[(8.77±4.52)vs(18.35±5.90)vs(30.67±5.47)ng/ml,P<0.05],T2DM组低于IGR组(P<0.05)。T2DM组、IGR组血浆GluT-4 mRNA及蛋白表达低于NGT组[(0.235±0.412)vs(3.386±0.698)vs(6.325±0.959),(1.235±0.092)vs(2.430±0.153)vs(4.843±0.299),P<0.05],T2DM组较IGR组降低(P<0.05)。T2DM组、IGR组血浆中SREBP-1C mRNA及蛋白表达水平高于NGT组[(7.570±1.233)vs(5.561±0.820)vs(2.880±0.483),(8.340±1.092)vs(5.279±0.798)vs(2.340±0.135),P<0.05],T2DM组较IGR组升高(P<0.05)。Pearson相关性分析显示,25(OH)D与血浆中GluT-4 mRNA、蛋白表达呈正相关(P<0.05),与HbA_1 c、HOMA-IR、FIns、FPG、2 hPG、TG及SREBP-1C mRNA、SREBP-1C蛋白表达呈负相关(P<0.05)。血浆中GluT-4表达与SREBP-1C表达呈负相关(P<0.05)。结论血浆25(OH)D降低可引发糖耐量异常,甚至导致糖尿病,其机制可能与血浆中GluT-4表达降低及SREBP-1C表达升高有关。
Objective To investigate the relationship between plasma 25-hydroxyvitamin D [25 (OH) D] and glucose transporter 4 (GluT-4) and cholesterol regulon binding protein 1C (SREBP-1C) in different glucose tolerance population. Methods Randomly divided into T2DM group, IGR group and normal control (NGT) group. Blood glucose, blood lipids, HbA1c, etc. were measured. Electrochemiluminescence was used to determine 25 (OH) D and FIns. The expression of GluT-4 and SREBP-1C protein was detected by Western blot. The expression of GluT-4 and SREBP-1C mRNA was detected by RT-PCR. Results 25 (OH) D in T2DM group and IGR group was lower than that in NGT group [(8.77 ± 4.52) vs (18.35 ± 5.90) vs (30.67 ± 5.47) ng / ml, P < <0.05). The mRNA and protein expression of GluT-4 in T2DM group and IGR group were lower than those in NGT group [(0.235 ± 0.412) vs (3.386 ± 0.698) vs (6.325 ± 0.959) vs (1.235 ± 0.092) vs (2.430 ± 0.153 vs ± 0.299), P <0.05]. T2DM group was lower than IGR group (P <0.05). The levels of SREBP-1C mRNA and protein in T2DM group and IGR group were significantly higher than those in NGT group [(7.570 ± 1.233) vs (5.561 ± 0.820) vs (2.880 ± 0.483 vs 8.340 ± 1.092 vs 5.279 ± 0.798 vs (2.340 ± 0.135), P <0.05]. T2DM group was higher than IGR group (P <0.05). Pearson correlation analysis showed that there was a positive correlation between 25 (OH) D and GluT-4 mRNA and protein in plasma (P <0.05), but not with HbA1c, HOMA-IR, FIns, FPG, 2 hPG, TG and SREBP- , SREBP-1C protein expression was negatively correlated (P <0.05). The plasma GluT-4 expression and SREBP-1C expression was negatively correlated (P <0.05). Conclusion The decrease of plasma 25 (OH) D may lead to impaired glucose tolerance and even diabetes. The mechanism may be related to the decrease of GluT-4 and the increase of SREBP-1C in plasma.