论文部分内容阅读
液相色谱中溶质计量置换保留模型的Ｚ值可用来对反相高效液相色谱中热变蛋白的分子构象进行表征。发现在异丙醇－甲酸４４％（Ｖ／Ｖ）－水体系为流动相时完全变性蛋白的Ｚ值随温度升高而降低，且Ｚ值与绝对温度倒数（１／Ｔ）成正比，因此蛋白分子构象变化可用Ｚ对１／Ｔ作图所得两条直线的斜率差值来表征，差值愈大，蛋白分子构象变化愈甚。该两直线的交点即为蛋白分子构象的突变温度。与通常采用的１ｇｋ＇对１／Ｔ的作图相比，用Ｚ对１／Ｔ作图对蛋白分子构象进行表征不仅对各种热变蛋白都有效，而且结果准确。
The Z value of the solute mass displacement retention model in liquid chromatography can be used to characterize the molecular conformation of thermoprotein in RP-HPLC. It was found that the Z value of fully denatured protein decreases with increasing temperature when the isopropanol-formic acid 44% (V / V) -water system is the mobile phase, and the Z value is proportional to the reciprocal of absolute temperature (1 / T) The conformational changes of protein molecules can be characterized by the difference between the slopes of the two lines obtained by plotting Z against 1 / T. The larger the difference is, the more conformational changes of protein molecules are. The intersection of these two lines is the mutation temperature of the conformation of the protein molecule. Characterization of the molecular conformation of a protein by Z vs. 1 / T is not only valid for a variety of thermolabels, but also accurate, compared to the commonly used 1 gk ’vs. 1 / T plot.