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分别用吸附交联法和共价键合法将葡萄糖氢化酶修饰在破碳电极表面上，并用循环伏安法和旋转圆盘电极技术研究其生物电催化动力学．提出酶修饰电极的界面模型并导出电极上酶促反应的稳态动力学表达式，从而指出由电化学方法测得的固定化酶表观动力学参数的物理意义．讨论了酶修饰电极的电而响应特性与固定化酶的性质、酶电极界面的几何特征、溶液中的传质以及电子传递体在电极基体上的电荷传递动力学等因素的关系．
Glucose hydrogenase was modified on the surface of carbon-broken electrode by adsorption-crosslinking method and covalent bonding method respectively. The bioelectrocatalytic kinetics was studied by cyclic voltammetry and spin disk electrode technique. The interfacial model of the enzyme-modified electrode was proposed and the steady-state kinetic expression of the enzymatic reaction on the electrode was derived. The physical meaning of the apparent kinetic parameters of the immobilized enzyme measured by the electrochemical method was pointed out. The relationship between the electrical response characteristics of the modified electrode and the properties of the immobilized enzyme, the geometric characteristics of the enzyme electrode interface, the mass transfer in solution and the charge transfer kinetics of the electron mediator on the electrode substrate was discussed.