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已知微型倒转重复转座因子 (MITEs)广泛分布于真核生物基因附近的非编码区。为探讨MITEs在基因组一级结构中的分布规律 ,通过对 82kb的水稻BAC克隆l1 3 3 2全序列分析 ,精确鉴定了 1 0个蛋白质编码基因的位置和转录方向以及 7个MITEs的位置 ,毫无例外的观察到 ,MITEs只要存在 ,总是位于最近的基因的上游 ,不管该基因与之相距多远、转录方向如何。另外还发现 ,1个MITE被 2个基因共享。由于MITEs易于鉴定 ,因而这种位置模式可以用作探测其下游基因存在的工具。
MITs are known to be widely distributed in non-coding regions near eukaryotic genes. In order to investigate the distribution of MITEs in the primary structure of the genome, the full-length sequence analysis of 82kb rice BAC clone l1 3 3 2 accurately identified the position and transcription direction of 10 protein coding genes and the location of 7 MITEs Without exception, it is observed that MITEs are always present upstream of the nearest gene, regardless of how far and far the gene is transcribed. In addition, one MITE was found shared by two genes. Because MITEs are easy to identify, this location pattern can be used as a tool to detect the presence of downstream genes.