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目的:探讨痛泻要方对溃疡性结肠炎(UC)模型大鼠IL-12及神经肽(NPY)的影响。方法:将SD大鼠随机分为空白组、模型组、柳氮磺嘧啶(SASP)组及痛泻要方高、中、低剂量组,每组各10只;采用TNBS/乙醇溶液灌肠法制备溃疡性结肠炎大鼠模型,运用ELISA法测定各组血清中细胞因子IL-12、神经肽Y(NPY)的含量。结果:与空白组比较,模型组血清IL-12、NPY水平均显著上升(P<0.01),各治疗组较模型组血清IL-12、NPY水平均明显下降(P<0.01)。结论:痛泻要方能通过下调IL-12、NPY的表达,调节肠道异常免疫反应,维持Th1、Th2的平衡,抑制炎症反应,修复溃疡,进而防治UC。
Objective: To investigate the effect of Tongxieyaofang on IL-12 and neuropeptide (NPY) in rats with ulcerative colitis (UC). Methods: SD rats were randomly divided into blank group, model group, sulfasalazine (SASP) group and TongxieYaoFang high, medium and low dose group, each group of 10; prepared by TNBS / ethanol enema The ulcerative colitis rat model was established. Serum levels of IL-12 and NPY were measured by ELISA. Results: Compared with the blank group, the levels of IL-12 and NPY in the model group increased significantly (P <0.01). The levels of IL-12 and NPY in the model group were significantly lower than those in the blank group (P <0.01). Conclusion: Tongxie Yaofang can regulate abnormal intestinal immune response, maintain the balance of Th1 and Th2, inhibit the inflammatory reaction, repair the ulcer, and then prevent and treat UC by down-regulating the expression of IL-12 and NPY.