近年来,国外学者在骨骼肌和心肌的研究中,大量采用去膜肌纤维标本和钙瞬变测量技术,由于这些技术的广泛应用,使人们在亚细胞水平对横纹肌力学性质的研究取得了重大进展,本文仅就这两类技术作一简要介绍。去膜肌纤维去膜肌纤维(skinned fiber)又称名取纤维(natory fiber),系指去除细胞膜的肌纤维。其最大特点是细胞膜的选择通透性消失。将去膜肌纤维置于相当细胞内液的灌流液中进行实验,可通过灌流液改变细胞内液的各种成分,故是研究肌肉生理和生化的理想标本。去膜肌纤维包括骨骼肌去膜肌纤维、心肌去膜肌纤维和平滑肌去膜 In recent years, foreign scholars in the study of skeletal muscle and myocardium extensive use of removal of muscle fiber specimens and calcium transient measurement techniques, due to the wide application of these technologies, so that people at the sub-cellular level on the mechanical properties of striated muscle has made significant progress This article only a brief introduction of these two types of technology. Skinning fibers, also known as natory fibers, are muscle fibers that remove the cell membrane. Its most prominent feature is the loss of cell membrane permeability. The removal of the muscle fibers in the intracellular fluid perfusion fluid experiments, perfusion fluid can change the various components of intracellular fluid, it is ideal for the study of muscle physiology and biochemistry specimens. Remodeling myofibers include skeletal muscle remover fibers, myocardial remover fibers and smooth muscle remover