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将小麦品种花培3号和豫麦57构建的DH群体的168个株系及其亲本,盆栽于两个环境中,利用324个SSR标记位点构建遗传图谱,对单叶净光合速率及相关参数、叶绿体色素含量和叶绿素荧光参数进行QTL定位和分析。利用基于混合线性模型的QTLNetwork 2.0,共检测到17个加性效应和20对上位性效应位点,其中所有加性效应位点和16对上位性效应位点具有环境互作效应。相关性较高的性状间有一些共同的QTL,表现出一因多效或者紧密连锁效应。在5D染色体上的Xwmc215至Xgdw63区段,检测到控制叶绿素a、叶绿素b和类胡萝卜素含量的3个主效QTL,各位点的遗传效应贡献率较大,增效基因均来源于花培3号,适用于分子标记辅助选择和聚合育种。另外,该区段与控制单叶净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)和胞间CO2浓度与胞外CO2浓度比值(Ci/Cr)的QTL的定位区间相近。位于5B染色体控制胞间CO2浓度的QTL是个微效基因,但是QTL与两种环境的互作效应表现的遗传贡献比较大。
168 lines and their parents of DH population constructed from the wheat cultivar Huapei 3 and Yumai 57 were potted in two environments and 324 SSR markers were used to construct the genetic map. The net photosynthetic rate and its correlation Parameters, chloroplast pigment content and chlorophyll fluorescence parameters QTL mapping and analysis. A total of 17 additive and 20 epistatic sites were detected using QTLNetwork 2.0 based on a mixed linear model. All additive sites and 16 pairs of epistatic sites had environmental interactions. There are some common QTL among the more relevant traits, showing a one-factor multi-effect or close-chain effect. Three main-effect QTLs controlling chlorophyll a, chlorophyll b and carotenoid content were detected in Xwmc215 to Xgdw63 on chromosome 5D, and the genetic contribution of each locus was larger. The efficiency-enhancing genes were all derived from flower bud 3 No. For molecular marker-assisted selection and polymerization breeding. In addition, the QTLs of Pn, Gs, Ci, and intercellular CO2 concentration and extracellular CO2 concentration (Ci / Cr) Similar range. The QTL controlling the intercellular CO2 concentration on chromosome 5B is a minor gene, but the genetic contribution of QTL to the interaction between the two environments is relatively large.