Zn isotope is a useful tool for tracing biogeochemical processes as zinc plays important roles in the biogeochemistry of natural systems.However,the Zn isotope composition in the lake eco-systems has not been well characterized.In order to resolve this problem,we investigate the Zn isotope compositions of suspended particulate matter(SPM)and biological samples collected from the Aha Lake and Hongfeng Lake,and their tributaries in summer and winter,aiming to explore the potential of this novel isotope system as a proxy for biogeochemical processes in aqueous environments.Concen-tration of dissolved Zn ranges from 0.65 to 5.06 μg/L and 0.74 to 12.04 μg/L for Aha Lake and Hongfeng Lake,respectively,while Zn(SPM)ranges from 0.18 to 0.70 mg/g and 0.24 to 0.75 mg/g for Aha Lake and Hongfeng Lake,respectively.The Zn isotope composition in SPM from Aha Lake and its main tributaries ranges from-0.18‰ to 0.27‰ and-0.17‰ to 0.46‰,respectively,and it varies from-0.29‰to 0.26‰ and-0.04‰ to 0.48‰,respectively in Hongfeng Lake and its main tributaries,displaying a wider range in tributaries than lakes.These results imply that Zn isotope compositions are mainly af-fected by tributaries inputting into Aha Lake,while adsorption process by algae is the major factor for the Zn isotope composition in Hongfeng Lake,and ZnS precipitation leads to the light Zn isotope com-position of SPM in summer.These data and results provide the basic information of the Zn isotope for the lake ecosystem,and promote the application of Zn isotope in biogeochemistry.