论文部分内容阅读
【目的】观察姜黄素(curcumin,Cur)对MPP+诱导的PC12细胞凋亡的保护作用,并且探讨其与iNOS关系。【方法】采用碘化丙啶(PI)染色流式细胞术(FCM)检测PC12细胞凋亡,间接免疫荧光FCM检测PC12细胞iNOS的表达。【结果】PC12细胞自然凋亡率为(1.5±0.1)%,0.5mmol·L-1MPP+作用24h后,PC12细胞凋亡率为(59.5±2.7)%;20μmol·L-1Cur和0.1mmol·L-1特异性iNOS抑制剂氨基胍(aminoguanidine,AG)对PC12细胞作用24h后无明显凋亡诱导作用,但分别使0.5mmol·L-1MPP+处理组细胞凋亡率由(59.5±2.7)%下降到(15.9±5.3)%和(39.7±8.7)%(P<0.01);PC12细胞经0.5mmol·L-1MPP+处理24h后,其iNOS表达率由正常对照的(13.5±1.5)%增加到(71.9±4.0)%(P<0.01),加入20μmol·L-1Cur同时处理24h后,其iNOS蛋白表达率由(71.9±4.0)%下降到(40.0±3.0)%(P<0.01)。【结论】Cur可以抑制MPP+诱导的PC12细胞凋亡,其作用机制之一可能与抑制iNOS高表达有关。
【Objective】 To observe the protective effect of curcumin (Cur) on apoptosis induced by MPP+ in PC12 cells and to explore its relationship with iNOS. 【Methods】 Apoptosis of PC12 cells was detected by propidium iodide (PI) staining and flow cytometry (FCM). Indirect immunofluorescence FCM was used to detect the expression of iNOS in PC12 cells. [Results] The natural apoptotic rate of PC12 cells was (1.5±0.1)%, and the apoptosis rate of PC12 cells was (59.5±2.7)% after treated with 0.5 mmol·L-1 MPP+ for 24 h; 20 μmol·L-1Cur and 0.1 mmol·L. The inhibitory effect of -1 specific iNOS inhibitor aminoguanidine (AG) on PC12 cells after 24 h did not induce apoptosis, but the apoptosis rate of cells treated with 0.5 mmol·L-1 MPP+ decreased from (59.5±2.7)%, respectively. It was (15.9±5.3)% and (39.7±8.7)% (P<0.01). After treated with 0.5mmol·L-1MPP+ for 24h, the iNOS expression rate of PC12 cells increased from (13.5±1.5)% to ( 71.9±4.0)% (P<0.01). After treated with 20 μmol·L-1Cur for 24 h, the expression rate of iNOS protein was decreased from (71.9±4.0)% to (40.0±3.0)% (P<0.01). [Conclusion] Cur can inhibit the apoptosis of PC12 cells induced by MPP+, and one of its mechanisms may be related to the inhibition of high expression of iNOS.