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对HPLC-MS/MS方法测定水中全氟辛酸(PFOA)和全氟辛烷磺酸(PFOS)过程中PFOA和PFOS峰面积的影响因素进行了研究。结果表明在一定的分析条件下PFOA和PFOS分别在保留时间2.08和2.33min处出现明显的色谱峰,随着样品的pH值升高,PFOA和PFOS峰面积逐渐增大,pH值为5.0的情况下,PFOA和PFOS峰面积达到极大值,随pH值增大呈现减小趋势。pH值5.0下PFOA峰面积比pH值1.5下PFOA峰面积大60.3%,相应的PFOS峰面积大32.5%。相同pH值情况下,PFOA、PFOS浓度与峰面积呈良好线性关系。水、甲醇、乙腈作为稀释溶剂对PFOA和PFOS峰面积没有明显影响。聚四氟乙烯、混合纤维等对PFOA和PFOS没有吸附,但尼龙滤膜对PFOA和PFOS有较强吸附。计算结果表明磺酸基与酰胺基团的结合能略大于羧基与酰胺基团的结合能,这可能是尼龙对PFOS吸附能力大于对PFOA吸附能力的原因之一。
The influence factors of PFOA and PFOS peak area in the determination of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) in water by HPLC-MS / MS method were studied. The results showed that PFOA and PFOS showed obvious peaks at 2.08 and 2.33min, respectively. Under the certain conditions, the peak area of PFOA and PFOS gradually increased with the increasing of the pH of the sample. The pH value was 5.0 , The peak areas of PFOA and PFOS reach the maximum value, showing a decreasing trend with the increase of pH value. The PFOA peak area at pH 5.0 is 60.3% larger than the pH 1.5 PFOA peak area, and the corresponding PFOS peak area is 32.5% larger. Under the same pH value, the concentration of PFOA and PFOS showed a good linear relationship with the peak area. Water, methanol, acetonitrile as a diluting solvent had no significant effect on the PFOA and PFOS peak areas. PTFE and mixed fiber did not adsorb PFOA and PFOS, but nylon membrane had strong adsorption on PFOA and PFOS. The calculated results show that the binding energy of sulfonic acid group and amide group is slightly larger than the binding energy of carboxyl group and amide group, which may be one of the reasons why nylon adsorbs PFOS more than PFOA.