论文部分内容阅读
目的:探讨细胞核转录因子(NF-κB)在胃癌、癌旁及其淋巴结组织中表达的临床意义,分析NF-κB的表达水平与临床病理特征和生存期以及原发灶和转移灶之间的关系.方法:用组织芯片和免疫组化的方法检测189例胃癌组织、54例癌旁组织、41例淋巴结转移灶、32例正常胃黏膜中NF-κB的表达.结果:胃癌、癌旁和正常胃黏膜NF-κB阳性表达率分别是75%,43%和16%.3组之间阳性表达率的差异具有显著性(P<0.05).NF-κB阳性表达与有无淋巴结转移、浸润深度、分化程度、Lauren分型有关(P<0.05).41例淋巴结转移灶和相对应的41例胃癌组织NF-κB阳性表达率差异具有显著性(P<0.05),转移灶中NF-κB高表达.单因素生存分析显示NF-κB阳性表达对胃癌患者生存期的影响有统计学意义(P<0.05),Cox多元回归分析显示NF-κB的异常表达可作为影响胃癌预后的独立因素(P<0.05).结论:NF-κB高表达参与胃癌的发生发展、浸润转移,是胃癌发生的早期事件,转移灶癌组织和原发灶相比有异质性.NF-κB的表达可以作为胃癌预后的独立指标.
Objective: To investigate the clinical significance of expression of nuclear factor-kappa B (NF-κB) in gastric cancer, paracancer and lymph node tissue, and to analyze the relationship between the expression of NF-κB and the clinicopathological characteristics, survival time, primary tumor and metastasis Methods: The expression of NF-κB in 189 cases of gastric cancer, 54 cases of paracancerous tissues, 41 cases of lymph node metastasis and 32 cases of normal gastric mucosa were detected by tissue microarray and immunohistochemistry.Results: The positive rates of NF-κB in gastric mucosa were 75%, 43% and 16%, respectively (P <0.05) .The positive expression rate of NF-κB was correlated with lymph node metastasis, depth of invasion (P <0.05). The positive expression rate of NF-κB in 41 cases of lymph node metastasis and 41 cases of gastric carcinoma was significantly different (P <0.05), while the expression of NF-κB (P <0.05). Cox multivariate regression analysis showed that the abnormal expression of NF-κB may be an independent factor affecting the prognosis of gastric cancer (P <0.05), and the single factor survival analysis showed that the positive expression of NF-κB had a significant effect on the survival of gastric cancer <0.05) .Conclusion: High expression of NF-κB is involved in the development of gastric cancer Invasion and metastasis, is an early event in gastric cancer, cancer metastasis and primary tumor expression heterogeneity .NF-κB can be used as an independent indicator of prognosis of gastric cancer compared.