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1960年,Bollum和Krakow在研究真核细胞DNA聚合酶时,几乎同时在两个实验室中发现了一种特殊的DNA聚合酶,它在缺乏模板的情况下,能够使底物聚合到核苷酸链3’-OH末端上,因而命名为末端转脱氧核苷酰酶(terminal deoxynucleotidyl transfer-ase,TdT),以区别复制的DNA聚合酶(α-、β-、γ-DNA聚合酶、病毒逆转录酶)。牛胸腺TdT的分子量为32,000,由分子量分别为24,000和8,000的β链和α链组成。最近发现人的TdT仅为一条高分子(分子量为62,000)
In 1960, when Bollum and Krakow studied eukaryotic DNA polymerases, they found a special type of DNA polymerase in almost two laboratories that, in the absence of a template, was able to polymerize substrates to nucleosides Acid 3’-OH terminus, hence the name terminal deoxynucleotidyl transfer-ase (TdT), to distinguish between replicating DNA polymerases (α-, β-, γ-DNA polymerase, Reverse transcriptase). The bovine thymus TdT has a molecular weight of 32,000 and consists of beta and alpha chains with molecular weights of 24,000 and 8,000, respectively. It has recently been found that human TdT is only a polymer (molecular weight of 62,000)