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目的 :研究中药福参 (AngelicamorriHayata)的活性成分。方法 :95 %乙醇提取、石油醚萃取、硅胶柱层析、重结晶等手段提取分离 ,波谱学方法及理化常数对照进行结构鉴定 ;采用K+ 、Ca2 + 诱导的大鼠主动脉环收缩模型对 3′S ( ) Ο 乙酰基亥茅酚和 (± ) 亥茅酚进行了活性测试。结果 :从石油醚部分依次分离到 4个色原酮类化合物 ,分别为 3′R (+) 亥茅酚 [3′R (+) hamaudol],3′S ( ) Ο 乙酰基亥茅酚 [3′S ( ) Ο acetylhamaudol],(± ) 亥茅酚 [(± ) hamaudol]和 3′S ( ) 亥茅酚 [3′S ( ) hamaudol]。 3′S ( ) Ο 乙酰基亥茅酚和 (± ) 亥茅酚对K+ 诱导的高钾模型呈剂量依赖型抑制效应。结论 :上述化合物均为首次从该植物中分离得到 ,(± ) 亥茅酚 [(± ) hamaudol]为一新天然产物 ;3′S ( ) Ο 乙酰基亥茅酚和 (± ) 亥茅酚通过抑制血管平滑肌细胞外钙内流而起到扩张血管的作用。
Objective: To study the active ingredients of AngelicamorriHayata. Methods: 95% ethanol extraction, petroleum ether extraction, silica gel column chromatography, recrystallization and other means extraction, spectral analysis and physical and chemical constants were used to identify the structure; K+ and Ca2+ induced rat aortic ring contraction model ’S () acetylcholine and (±) haloxyl were tested for activity. RESULTS: Four chromogenic ketone compounds were isolated from the petroleum ether fractions. They were 3’R (+) phenolic [3’R (+) hamaudol] and 3’S () acetylcholine. 3′S () Ο acetylhamaudol], (±) haloxyphenol ((±) hamaudol) and 3′S () haloxyphene [3′S () hamaudol]. 3′S(), acetylcholine and (±) halothane have a dose-dependent inhibitory effect on the K+ induced potassium model. Conclusion: The above compounds were isolated from this plant for the first time. (±) hamaudol is a new natural product; 3′S () is acetylcholine and (±) halothol. It acts to dilate blood vessels by inhibiting extracellular calcium influx from vascular smooth muscle.