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目的:探讨黄芪注射液对镉致孕鼠毒性的拮抗作用。方法:将受孕Sprague Dawley(SD)大鼠随机分成3组:黄芪注射液+氯化镉组(A组)、氯化镉组(Cd组)和对照组(C组)。A、Cd组分别每天饮用含40 mg/L氯化镉的水,C组饮用等量蒸馏水;A组每天腹腔注射黄芪注射液10 g/kg/d,Cd组和C组每天腹腔注射等量蒸馏水;各组每天均喂食普通饲料。20天的孕鼠麻醉后心脏取血,测定血清镉(Cd)、谷胱甘肽过氧化物酶(glutathiOne Pe roxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutas,SOD)和相关性激素(雌激素E2孕激素P)含量,并取胎盘组织冷冻保存测其中镉含量。结果:与Cd组(0.68±0.12、0.23±0.04、3.45±0.68、1.78±0.09、11.73±0.68、22.97±1.07)相比,A组孕鼠血清镉(0.23±0.04)、胎盘镉含量(0.12±0.03)下降(P<0.05),血清中GSH-Px、SOD、E2、P含量(12.57±2.10、7.47±1.35、18.36±0.96、40.75±1.27)升高(P<0.05)。结论:黄芪注射液可拮抗镉对孕鼠的毒性,作用机制可能与其有效成分清除自由基和抗氧化作用等有关。
Objective: To investigate the antagonistic effect of Astragalus injection on the toxicity of cadmium to pregnant rats. Methods: Sprague Dawley (SD) rats were randomly divided into three groups: astragalus injection + cadmium chloride group (A group), cadmium chloride group (Cd group) and control group (C group). Groups A and C each received 40 mg / L cadmium chloride water daily and Group C drinking the same amount of distilled water. Group A received daily intraperitoneal injection of astragalus injection 10 g / kg / d, and groups Cd and C received daily intraperitoneal injections Distilled water; each group were fed normal feed everyday. Twenty-day-old pregnant rats were anesthetized and their hearts were taken blood for determination of serum cadmium (Cd), glutathiOne Pe roxidase (GSH-Px), superoxide dismutas (SOD) and related hormones (Estrogen E2 progesterone P) content, and take placental tissue to measure the content of cadmium in it. Results: Compared with Cd group (0.68 ± 0.12,0.23 ± 0.04,3.45 ± 0.68,1.78 ± 0.09,11.73 ± 0.68,22.97 ± 1.07), serum cadmium (0.23 ± 0.04), placental cadmium content (0.12 (P <0.05). The content of GSH-Px, SOD, E2 and P in serum increased (12.57 ± 2.10,7.47 ± 1.35,18.36 ± 0.96,40.75 ± 1.27) (P <0.05). Conclusion: Astragalus injection can antagonize the toxicity of cadmium to pregnant rats, the mechanism may be related to its active ingredients scavenging free radicals and antioxidant effects.