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[目的]探讨SD大鼠新生期苯并芘暴露对抗氧化酶活性和精子生成量的影响。[方法]自大鼠出生后第1天起连续7d给予0、5、10和25mg/kg 3,4-苯并芘灌胃,在出生后第8、35和90天解剖大鼠,脏器称重并计算脏器系数和每日精子生成量。测定睾丸中(出生后第8、35和90天)CYP1A1基因表达和超氧化物歧化酶(superoxide dismutase,SOD)及过氧化氢酶(catalase,CAT)活性。[结果]连续染毒7d,在出生后第8天,睾丸中细胞色素P450氧化酶基因(CYP1A1基因)表达随暴露剂量升高而上升,且10和25mg/kg暴露组基因表达量与对照组相比,差异均有统计学意义(P<0.05);在出生后第35和90天时,各组睾丸中CYP1A1基因几乎没有表达。SOD、CAT活性测定显示:在出生后第8天,与对照组比,25mg/kg组的SOD活性上升(P<0.05),虽然各暴露组CAT活性与对照组相比差异无统计学意义,但是随着剂量的增大,CAT活性呈上升趋势;出生后第35天时,与对照组相比,各剂量组SOD、CAT活性均明显下降(P<0.05);在出生后第90天,SOD、CAT活性变化与对照组比较,差异无统计学意义。出生后第90天时,苯并芘10和25mg/kg暴露组每日精子生成量较对照组下降(P<0.05)。[结论]SD大鼠新生期暴露苯并芘可导致成年期睾丸每日精子生成量下降,影响睾丸抗氧化酶SOD和CAT的活性。
[Objective] To investigate the effects of neonatal benzopyrene exposure on antioxidant enzyme activity and sperm production in SD rats. [Method] The rats were gavaged with 0, 5, 10 and 25 mg / kg 3,4-benzopyrene for 7 days after birth on the first day after birth and were dissected on days 8, 35 and 90 Weighing and counting organ coefficients and daily sperm production. The CYP1A1 gene expression and superoxide dismutase (SOD) and catalase (CAT) activities in the testes (days 8, 35 and 90 after birth) were determined. [Result] The expression of cytochrome P450 oxidase gene (CYP1A1) in testis increased with the increase of exposure dose on the 8th day after birth, and the expression of CYP1A1 gene in testis at 10 and 25 mg / kg exposure was significantly higher than that of the control group (P <0.05). At the 35th and 90th days after birth, there was almost no expression of CYP1A1 in testes. The activity of SOD and CAT increased in the 25 mg / kg group compared with the control group on the 8th day after birth (P <0.05). Although there was no significant difference in the activity of CAT between the exposed groups and the control group, However, the activity of CAT increased with the dose increasing. On the 35th day after birth, compared with the control group, the activity of SOD and CAT in each dose group decreased significantly (P <0.05). On the 90th day after birth, , CAT activity changes compared with the control group, the difference was not statistically significant. At the 90th day after birth, the daily sperm production of benzopyrene 10 and 25 mg / kg exposure groups was lower than that of the control group (P <0.05). [Conclusion] Benzo [a] pyrene exposure in neonatal SD rats can lead to the decrease of daily sperm production in adult testes and the activity of antioxidant enzymes SOD and CAT in testis.