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通过研究硒对端粒酶活性和端粒长度的作用 ,探讨硒抗衰老的生物学机制。实验以人肝细胞株L 0 2为研究对象 ,分别补充 0 .5和 2 .5 μmol L亚硒酸钠 ,采用端粒重复序列扩增 焦磷酸根酶联发光法、逆转录聚合酶链式反应法及流式荧光原位杂交法 ,分别检测细胞的端粒酶活性、人端粒酶逆转录酶催化亚基基因 (hTERT)的表达及端粒长度的变化。结果表明 :常规培养的肝细胞株L 0 2的端粒酶活性和hTERT基因表达水平均较低。补充 0 .5和2 .5 μmol L亚硒酸钠三周后细胞生长状况良好、端粒酶活性和hTERT基因表达水平显著性增高 ,且呈一定的剂量 效应关系。细胞补充亚硒酸钠四周后端粒长度显著增长。说明营养浓度的亚硒酸钠可通过提高端粒酶活性和增长端粒长度来减缓L 0 2肝细胞衰老、延长细胞寿命。
By studying the effect of selenium on telomerase activity and telomere length, the biological mechanism of anti-aging of selenium was explored. In the experiment, human hepatocyte cell line L 0 2 was used as the research object, respectively 0.5 and 2.5 μmol L sodium selenite were supplemented with telomeric repeat amplification pyrophosphate enzyme-linked luminescence, reverse transcription polymerase chain reaction The expression of telomerase reverse transcriptase catalytic subunit gene (hTERT) and telomere length were detected by flow cytometry, flow cytometry and flow cytometry. The results showed that the telomerase activity and hTERT gene expression of L 0 2 in normal cultured hepatocyte cell line were low. Three weeks after supplementation of 0.5 and 2.5 μmol L sodium selenite, cell growth was good, and telomerase activity and hTERT gene expression were significantly increased, showing a dose-dependent effect. Telomere length was significantly increased after cells were supplemented with sodium selenite for four weeks. Sodium selenite at a nutrient concentration could slow down L 0 2 hepatocyte senescence and prolong cell life by increasing telomerase activity and increasing telomere length.