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目的 :探索白细胞介素 6 (interleukin 6 ,IL 6 )增强乳腺癌细胞表达乳腺癌抗原 (CA15 3)和癌胚抗原 (CEA)的作用。方法 :将含IL 6蛋白编码顺序1176bpcDNA插入Pci neo哺乳动物表达载体。将重组载体转染MCF 7乳腺癌细胞 ,采用ELASA方法测定细胞培养上清液内IL 6浓度 ,用MEIA(microp articalenzymeimmunoassay)方法测定上清液中肿瘤相关抗原CA15 3、CEA和CA12 5。结果 :含有外源IL 6基因的MCF 7细胞分泌IL 6浓度 (338.5±2 2 .6pg·10 -6细胞 )明显高于父本没有含外源基因的MCF 7细胞 (2 5 .4± 4 .6pg·10 -6细胞 )和仅含空载体Pci neo的MCF 7细胞 (19.6± 3.0pg·10 -6细胞 ) (P<0 .0 1)。细胞培养d 3后 ,带外源IL 6基因的MCF 7细胞培养上清液中CA15 3和CA12 5水平 (分别为 14 .9± 2 .3和 38.8± 5 .1μg·10 -6细胞 )明显高于父本 (分别为 6 .6± 1.5和 10 .0± 1.6 μg·10 -6细胞 )和空载体Pci neo的MCF 7细胞 (分别为 3.4±0 .7和 14 .6± 2 .2 μg·10 -6细胞 ,P <0 .0 5 )。而转染IL 6基因没有明显改变CEA表达 (P >0 .0 5 )。结论 :IL 6具有诱导肿瘤相关抗原的表达和增强肿瘤细胞的免疫原性的作用 ,提示IL 6可能增强机体对肿瘤的免疫反应性。
Objective: To explore the role of interleukin 6 (IL 6) in enhancing the expression of breast cancer antigen (CA15 3) and carcinoembryonic antigen (CEA) in breast cancer cells. Methods: The 1176 bp cDNA containing IL6 protein coding sequence was inserted into Pci neo mammalian expression vector. The recombinant vector was transfected into MCF7 breast cancer cells, and the concentration of IL-6 in the cell culture supernatant was measured by ELASA method. The tumor-associated antigens CA15 3, CEA and CA12 5 in the supernatant were measured by MEIA method. Results: The concentration of IL-6 secreted by MCF-7 cells containing exogenous IL-6 gene (338.5 ± 2.26pg · 10-6 cells) was significantly higher than that of MCF-7 cells without exogenous gene (2.54 ± 4 .6 pg · 10 -6 cells) and MCF 7 cells (19.6 ± 3.0 pg · 10 -6 cells) containing only empty vector Pci neo (P <0.01). The levels of CA15 3 and CA12 5 in supernatants of MCF 7 cells with exogenous IL 6 gene (14.9 ± 2. 3 and 38.8 ± 5 .1 μg · 10 -6, respectively) were significantly higher after d 3 culture MCF 7 cells (3.4 ± 0.7 and 14.6 ± 2.2, respectively, higher than the paternal (6.6 ± 1.5 and 10.0 ± 1.6 μg · 10 -6 cells) and the empty vector Pci neo μg · 10 -6 cells, P <0.05). The transfection of IL 6 gene did not change the CEA expression (P> 0.05). Conclusion: IL 6 can induce tumor-associated antigen expression and enhance the immunogenicity of tumor cells, suggesting that IL 6 may enhance the body’s immune response to tumors.