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通过杏鲍菇多孢分离菌株的ISSR、RAPD分子标记和体细胞不亲和性分析,体细胞不亲和性分析得出子代杏1、杏3、杏4与出发菌株湘杏98有拮抗线,杏1、杏2、杏3、杏4四株子代均有明显的拮抗线。16条ISSR引物和19条RAPD引物分别扩增出99、154个条带,多态性条带分别占48.48%、61.00%;相似系数变异范围分别为0.63~0.86、0.57~0.83。湘杏98、杏2、杏3在2种标记的聚类结果上存在差异。体细胞不亲和性分析从表型上(拮抗线有无)反应了菌株的遗传变异。RAPD标记遗传相似系数小于ISSR标记,表明ISSR标记更适合亲缘关系较近的种群间遗传多样性分析。
ISSR, RAPD molecular markers and somatic incompatibility analysis of the strains isolated from Pleurotus eryngii showed that progeny apricot 1, apricot 3 and apricot 4 were antagonistic to the starting strain Xiangxing 98 by somatic incompatibility analysis Line, apricot 1, apricot 2, apricot 3, apricot 4 four offspring have obvious antagonistic line. A total of 99,154 bands were amplified by 16 ISSR primers and 19 RAPD primers, respectively. The polymorphic bands accounted for 48.48% and 61.00% respectively. The range of similarity coefficient was 0.63-0.86 and 0.57-0.83, respectively. Xiangxing 98, Xing 2, Xing 3 differences in the results of the two kinds of markers clustering. Analysis of somatic incompatibility reflects the genetic variation of the strain phenotypically (antagonistic line presence). The genetic similarity coefficient of RAPD markers was less than that of ISSR markers, indicating that ISSR markers are more suitable for genetic diversity analysis among populations with closer genetic relationship.