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目的：分析研究实时荧光定量聚合酶链式反应（FQ-PCR）对解脲支原体、沙眼衣原体的检测结果，以了解本地区在不同性别及年龄段的感染情况。方法选取1000例疑似非淋菌性尿道炎患者，均采取FQ-PCR进行检测，统计分析不同男女性别及年龄段的阳性检出率。结果 UU和Ct的检出率为44.50%，其中在女性患者中的检出率（55.31%）要明显高于男性（35.58%）（P<0.05）。其中女性患者UU的检出率（37.61%）显著高于男性（18.25%），差异具有统计学意义（P0.05）。18~40岁患者的阳性率（81.35%）显著高于年龄大于40岁患者（18.65%），差异显著（P<0.05）。结论 FQ-PCR对UU、Ct的检出率较高，本地区以女性患者及18~40岁年龄段的检出率较高，应加大对上述人群的监管力度。“,”Objective Analysis of real-time fluorescence quantitative polymerase chain reaction (FQ-PCR)of Ureaplasma urealyticum and Chlamydia trachomatis detection results, in order to understand the region in different gender and age of infection. Methods A total of 1000 cases of suspected patients with nongonococcal urethritis, FQ-PCR detection. Statistical analysis of different gender and age of the positive rate. Results The detection rate of UU and Ct was 44.50%, among which the detection rate (55.31%) in female patients was significantly higher than that in males (35.58%)(P<0.05).The detection rate of UU(37.61%)in female patients was significantly higher than that in male (18.25%), and the difference was statistically significant(P0.05). The positive rate (81.35%) of 18-40 years old patients was significantly higher than that in patients older than 40 years (18.65%), and the difference was significant (P<0.05). Conclusion The detection rate of UU in FQ-PCR and Ct is higher, the detection rate of female patients in the local area and the age of 18~40 years old is higher, and the supervision of these people should be increased.