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目的探讨Survivin基因在结直肠癌中的表达及其意义。方法应用免疫组织化学(SP)法和TdT介导的dUTP缺口末端标记技术原位观察48例结直肠癌患者的癌组织、癌旁组织和正常组织中Survivin蛋白的表达和细胞凋亡。结果癌组织Survivin的阳性表达相对含量(PU)值(38.76±5.14)明显高于癌旁(25.17±7.26)和正常组织(0.57±0.03),差异有统计学意义(t=2.54,P<0.05),正常大肠黏膜中极少见Survivin蛋白表达;癌旁和癌组织凋亡指数(AI)分别为(7.51±2.63)%和(4.65±1.76)%,两者差异有统计学意义(t=2.18,P<0.05);Survivin蛋白的阳性表达与患者的性别、年龄、肿瘤大小无相关性(P>0.05),与癌组织的分化程度、淋巴结转移和Dukes分期相关,低分化、有淋巴结转移和DukesC/D期的Survivin蛋白表达显著高于相关组别(P<0.05)。结论Survivin在结直肠癌组织中表达上调,Survivin表达可抑制结直肠癌的细胞凋亡,这可能是结直肠癌发生、发展的有关因素;Survivin的表达与结直肠癌的恶性生物学行为有关。
Objective To investigate the expression of Survivin gene in colorectal cancer and its significance. Methods Survivin protein expression and apoptosis in 48 cases of colorectal cancer tissues, adjacent tissues and normal tissues were observed by immunohistochemistry (SP) and TdT-mediated dUTP nick end labeling. Results The positive expression of Survivin (38.76 ± 5.14) in cancer tissue was significantly higher than that in adjacent normal tissue (25.17 ± 7.26) and normal tissue (0.57 ± 0.03), the difference was statistically significant (t = 2.54, P <0.05 ). Survivin protein expression was extremely rare in normal colorectal mucosa. The apoptotic index (AI) in paracancerous and cancerous tissues were (7.51 ± 2.63)% and (4.65 ± 1.76)% respectively, with significant difference between the two groups (t = 2.18, P <0.05). There was no correlation between the positive expression of Survivin protein and the gender, age and tumor size (P> 0.05). The expression of Survivin protein was correlated with the degree of differentiation, lymph node metastasis and Dukes stage, lymph node metastasis And Dukes C / D phase Survivin protein expression was significantly higher than the relevant group (P <0.05). Conclusions Survivin is up-regulated in colorectal cancer tissues, and Survivin expression may inhibit the apoptosis of colorectal cancer. This may be related to the occurrence and development of colorectal cancer. Survivin expression is related to the malignant behavior of colorectal cancer.