论文部分内容阅读
对５４例甲状腺癌标本分别用ＤＮＡ聚合酶链反应－限制性片段长度多态性分析（ＰＣＲ－ＲＦＬＰ）法检测ｐ５３基因第２４８位密码子的突变及免疫组化法检测ｐ５３蛋白，并用时序检验分析ｐ５３基因突变和ｐ５３蛋白表达与甲状腺癌的关系。结果发现，甲状腺癌中１５例（２７８％）有ｐ５３基因突变和３０例（５５６％）有ｐ５３蛋白过度表达，临床分期晚、分化程度低的甲状腺癌突变率较高，ｐ５３抑癌基因突变和ｐ５３蛋白阳性的病例有较高的复发率和死亡率。表明ｐ５３基因突变及其蛋白过度表达在甲状腺癌的发生和发展过程中起重要作用，是预后不良的标志。
The mutation of the 248th codon of p53 gene was detected by PCR-RFLP in 54 cases of thyroid cancer specimens, and the p53 protein was detected by immunohistochemistry. The relationship between p53 gene mutation and p53 protein expression and thyroid cancer was analyzed. The results showed that 15 cases (27.8%) of thyroid cancer had p53 gene mutation and 30 cases (55.6%) had p53 protein overexpression. The late stage of clinical stage and low degree of differentiation of thyroid cancer had higher mutation rate and p53 inhibition. Oncogene mutations and p53-positive cases have higher recurrence rates and mortality. It indicates that p53 gene mutation and overexpression of protein play an important role in the occurrence and development of thyroid cancer, and it is a sign of poor prognosis.