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报道了仿刺参 (ApostichopusjaponicusLiao)多倍体诱导的结果。采用经紫外线照射后的海水成功地使仿刺参分别单独产卵、排精 ,从而准确地控制了仿刺参的多倍体诱导的开始时间。使用 0 2、 0 4mg/L细胞松弛素B (CB)在授精后 5、 1 0min开始处理受精卵抑制第一极体 (PB1 )放出 ,用 1 0、 2 0、 30、 40mg/L的 6-二甲基氨基嘌呤 ( 6-DMAP)分别在授精后 5、 1 0、 2 0、 2 8、 35min开始处理抑制受精卵PB1 、第二极体(PB2 )放出的方法诱导了刺参的多倍体。研究了诱导的药物浓度、处理时间与诱导效果的关系 ,同时对幼体的成活率等进行了探讨。结果表明 ,2种药物均可诱导仿刺参产生三倍体和四倍体。采用CB抑制PB1 诱导 ,到达小耳幼体时 ,可产生 2 1 3%的四倍体。用 6-DMAP抑制PB2 放出诱导三倍体 ,三倍体率可达 2 5 8%。在幼虫浮游期中 ,处理组和对照组幼虫的摄食、生长、浮游时间以及变态率等方面没有明显差异。
Reported the results of polyploid induction of Apostichopus japonicus Liao. Using seawater after UV irradiation succeeded in spawning separately, so as to accurately control the start time of polyploid induction of the sea cucumber. Using 0 2, 0 4mg / L cytochalasin B (CB) at fertilization after 5,10 min start processing fertilized egg inhibit the first polar body (PB1) release, with 10,2, 30,40 mg / L of 6 -dimethylaminopurine (6-DMAP), respectively, inhibited the release of PB1 and the second polar body (PB2) from fertilized eggs at 5, 10, 20, 28 and 35 min after insemination Ploidy. The relationship between induced drug concentration, treatment time and induction effect was studied, and the survival rate of larvae was also discussed. The results showed that two kinds of drugs can induce Ipomoea simia to produce triploids and tetraploids. Using CB to inhibit the induction of PB1, it reached 21.3% of the tetraploid when it reached the small ear. PB6 release induced by 6-DMAP induced triploid, triploid rate up to 25.8%. There was no significant difference in feeding, growth, lag time and rate of metamorphosis in the larvae during the larval period.