本文在利用微晶纤维素—硅胶G薄层板对氨基酸获得了良好的分离基础上,进一步探索了用亚双向分离外标原位扫描定量测定双向薄层色谱上的氨基酸含量的可行性。结果表明此方法较方便可靠,重复性好。(亚双向分离外标校正,就是外标混合液点,一向充分展开分离,而另一向只展开短距离,约1厘米左右)。 Based on the good separation of amino acids from microcrystalline cellulose-silica gel G plate, the feasibility of quantitative determination of amino acid in two-dimensional thin layer chromatography by sub-bidirectional external standard in situ scanning was further explored. The results show that this method is more convenient and reliable, good repeatability. (Sub-bidirectional external standard calibration, is the external standard mixture point, has been fully developed separation, and the other to only start a short distance, about 1 cm).